Rat L-Selectin ELISA kit

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Associated names

Sell; Lnhr; Ly-22; L-selectin; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; CD antigen CD62L

Abbreviation

SELL

Uniprot No.

P30836

Species

Rattus norvegicus (Rat)

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Stem Cells

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat L-Selectin/CD62L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	105
	Range %	99-112
1:2	Average %	93
	Range %	88-99
1:4	Average %	102
	Range %	96-107
1:8	Average %	88
	Range %	83-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.979	2.829	2.904	2.799
2500	1.869	1.941	1.905	1.801
1250	1.302	1.249	1.275	1.171
625	0.833	0.877	0.855	0.750
312	0.585	0.603	0.594	0.489
156	0.350	0.349	0.349	0.245
78	0.228	0.231	0.230	0.125
0	0.103	0.106	0.105

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Rat L-Selectin ELISA kit is a tool for detecting L-Selectin in your samples. Designed specifically for Rattus norvegicus, this ELISA kit is ideal for researchers studying stem cells. With a detection range of 78pg/ml-5000pg/ml and a sensitivity of 39pg/ml, you can be confident that this kit will provide accurate and reliable results.

This kit is versatile and can be used with a variety of sample types, including serum, plasma, and tissue homogenates. With an assay time of just 1-5 hours and a sample volume of 50-100ul, this ELISA kit is both efficient and convenient.

The Rat L-Selectin ELISA kit is easy to use and comes complete with all necessary components. Simply follow the step-by-step instructions to obtain precise and reproducible results. The detection wavelength of 450 nm ensures that you can easily measure the concentration of L-Selectin in your samples. Its ease of use and quick assay time make it a convenient and efficient choice for any laboratory.

Neuro-glial and systemic mechanisms of pathological responses in rat models of primary blast overpressure compared to "Composite" Blast Stanislav I. Svetlov et al,Frontiers in Neurology,2012

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Selectin/LECAM family

Tissue Specificity

Expressed in peripheral blood mononuclear cells (PBMC), spleen and thymus.

Database Links

STRING: 10116.ENSRNOP00000003733

UniGene: Rn.10461

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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