Rat Leukotriene B4,LT-B4 ELISA Kit

Leukotriene B4 (LT-B4) is a potent lipid mediator derived from arachidonic acid through the 5-lipoxygenase pathway. This bioactive eicosanoid plays important roles in inflammatory responses, serving as a powerful chemoattractant for neutrophils and other immune cells. LT-B4 is involved in various pathological conditions including asthma, arthritis, and inflammatory bowel disease, making it an important biomarker for studying inflammatory processes and immune system activation in research applications.

The Rat Leukotriene B4,LT-B4 ELISA Kit (CSB-E08035r) is designed for quantitative measurement of LT-B4 in Rattus norvegicus samples. This sandwich-based assay accommodates serum, plasma, and tissue homogenates with a detection range of 15.6 pg/mL to 1000 pg/mL and sensitivity of 3.9 pg/mL. The assay requires 50-100 μL sample volume, completes within 1-5 hours, and uses detection at 450 nm wavelength for reliable quantification of rat LT-B4 levels.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining inflammatory responses and arachidonic acid metabolism pathways. Studies have applied the kit to quantify leukotriene levels in various biological samples including serum and tissue homogenates from rat models.

• Inflammatory disease models: Measurement of leukotriene levels in ammonia-induced acute pharyngitis studies, quantifying inflammatory mediators alongside pro-inflammatory cytokines in pharyngeal tissue
• Serum biomarker analysis: Quantification of leukotriene concentrations in serum samples as part of comprehensive inflammatory marker panels that include immunoglobulin and histamine measurements
• Arachidonic acid metabolism research: Analysis of leukotriene levels in liver tissue as part of broader studies examining arachidonic acid derivative profiles and lipid mediator pathways