Rat Leukotriene B4,LT-B4 ELISA Kit

Code CSB-E08035r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Leukotriene B4,LT-B4
Alternative Names
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
3.9 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 94    
Range % 89-99    
1:2 Average % 97    
Range % 91-103    
1:4 Average % 97    
Range % 92-104    
1:8 Average % 93    
Range % 88-97    
The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 92 87-96    
EDTA plasma (n=4) 97 93-106    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pg/ml OD1 OD2 Average Corrected    
1000 2.442 2.541 2.492 2.320    
500 1.887 1.986 1.937 1.765    
250 1.282 1.263 1.273 1.101    
125 0.830 0.821 0.826 0.654    
62.5 0.511 0.502 0.507 0.335    
31.2 0.370 0.361 0.366 0.194    
15.6 0.264 0.259 0.262 0.090    
0 0.171 0.172 0.172      
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Rat LT-B4 ELISA Kit was designed for the quantitative measurement of Rat LT-B4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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