Rat Lipopolysaccharides(LPS) ELISA Kit

CUSABIO's rat Lipopolysaccharides(LPS) ELISA kit can assay for rat LPS in the serum, plasma, and tissue homogenates. This assay uses the sandwich ELISA to quantitate LPS in samples. Standards and samples are pipetted into the wells. A highly specific biotin-conjugated LPS antibody is subsequently added to wells. After washing, HRP-avidin conjugate is added to the wells. Following a thorough washing, the TMB substrate solution is added to the wells, making the solution turns blue due to the enzyme reaction catalyzed by HRP. The solution turns blue turns yellow after the addition of the stop solution. The color intensity is measured on a microplate reader at 450nm and is proportional to the amount of LPS captured in the initial step.

This kit provides a detection range of 0.156 ng/mL-10 ng/mL and has high sensitivity as low as 0.039 ng/mL. It has also been validated on several parameters, including precision, linearity, and recovery.

LPS is a large glycolipid containing lipid A, the core oligosaccharide, and the O antigen. It is a major structural component of the outer membrane (OM) of Gram-negative bacteria. LPS transforms the OM into an effective permeability barrier, protecting the membrane from certain kinds of antimicrobial compounds attack. It also plays a critical role in bacteria-host interactions by regulating responses through the immune system.