Rat Lipopolysaccharides(LPS) ELISA Kit

Instructions
Code CSB-E14247r
Size 96T,5×96T,10×96T
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Product Details

Target Name Lipopolysaccharides(LPS)
Abbreviation LPS
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-100  
1:2 Average % 102  
Range % 96-107  
1:4 Average % 98  
Range % 90-106  
1:8 Average % 88  
Range % 82-104  
Recovery
The recovery of rat LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 84-100  
EDTA plasma (n=4) 104 100-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 1.987 1.999 1.993 1.819  
5 1.395 1.357 1.376 1.202  
2.5 0.887 0.897 0.892 0.718  
1.25 0.595 0.576 0.586 0.412  
0.625 0.391 0.384 0.388 0.214  
0.312 0.301 0.324 0.313 0.139  
0.156 0.247 0.261 0.254 0.080  
0 0.173 0.175 0.174    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

I am interested in your kit Rat Lipopolysaccharides, LPS ELISA Kit (CSB-E14247r) (24 strip-well).
Taking into consideration the number of standards, how many samples can you analyse? Do you need to do this in duplicate?

A:
Thanks for your inquiry.
CSB-E14247r
For this target, dilution is not needed if you test serum and plasma samples. We validated normal serum before and the test value is ND--0.25ng/ml. If you test samples of other modes, you can refer to the research situation of this mode and judge if pretest is needed or not. Take 24T as an example, if you run single well for the standard and the sample, you can test 16 samples. You can also run duplicated wells to reduce errors during the assay.
Pls let me know if you have any further questions. Thank you.
 Customer Reviews

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