Rat Lipopolysaccharides(LPS) ELISA Kit

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Rat Lipopolysaccharides(LPS) ELISA Kit

Name: Rat Lipopolysaccharides(LPS) ELISA Kit
Brand: CUSABIO
SKU: CSB-E14247r

^Instructions

Code	CSB-E14247r
Size	96T,5×96T,10×96T
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Target Name

Lipopolysaccharides(LPS)

Abbreviation

LPS

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	102
	Range %	96-107
1:4	Average %	98
	Range %	90-106
1:8	Average %	88
	Range %	82-104

Recovery

The recovery of rat LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-100
EDTA plasma (n=4)	104	100-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	1.987	1.999	1.993	1.819
5	1.395	1.357	1.376	1.202
2.5	0.887	0.897	0.892	0.718
1.25	0.595	0.576	0.586	0.412
0.625	0.391	0.384	0.388	0.214
0.312	0.301	0.324	0.313	0.139
0.156	0.247	0.261	0.254	0.080
0	0.173	0.175	0.174

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Xylooligosaccharide Modulates Gut Microbiota and Alleviates Colonic Inflammation Caused by High Fat Diet Induced Obesity Y Fei,Front Physiol,2020
TNF-a induces Drp1-mediated mitochondrial fragmentation during inflammatory cardiomyocyte injury Shen YL.et al,Int J Mol Med,2018
Beneficial effects of soy milk and fiber on high cholesterol diet-induced alteration of gut microbiota and inflammatory gene expression in rats. Lee SM.et al,Food Funct.,2015

■ Q&A

I am interested in your kit Rat Lipopolysaccharides, LPS ELISA Kit (CSB-E14247r) (24 strip-well).
Taking into consideration the number of standards, how many samples can you analyse? Do you need to do this in duplicate?

Thanks for your inquiry.
CSB-E14247r
For this target, dilution is not needed if you test serum and plasma samples. We validated normal serum before and the test value is ND--0.25ng/ml. If you test samples of other modes, you can refer to the research situation of this mode and judge if pretest is needed or not. Take 24T as an example, if you run single well for the standard and the sample, you can test 16 samples. You can also run duplicated wells to reduce errors during the assay.
Pls let me know if you have any further questions. Thank you.

■ Customer Reviews

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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