Human Lipopolysaccharides,LPS ELISA Kit

Code CSB-E09945h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
1.56 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 92  
Range % 87-96  
1:8 Average % 94  
Range % 89-99  
The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-97  
EDTA plasma (n=4) 88 82-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.809 2.960 2.885 2.720  
200 2.227 2.366 2.297 2.132  
100 1.515 1.556 1.536 1.371  
50 0.789 0.755 0.772 0.607  
25 0.432 0.470 0.451 0.286  
12.5 0.303 0.325 0.314 0.149  
6.25 0.235 0.232 0.234 0.069  
0 0.164 0.165 0.165    
Materials provided
  • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
  • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
  • Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
  • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
  • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
  • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
  • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
  • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
  • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human LPS ELISA Kit was designed for the quantitative measurement of Human LPS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .


Customer Reviews and Q&A

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I would like to know if this ELISA kit is SPECIFIC for LPS of Escherichia Coli
I mean CSB-E09945h ELISA kit

Thanks for your inquiry.
CSB-E09945h detects the residual LPs in the human body due to infection.Pls let me know if you have any further questions. Thank you.

Could you share with me what the antibodies in this kit bind to CSB-E09945h?

Thanks for your inquiry.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.Pls let me know if you have any further questions. Thank you.

I have an interest in knowing product information "Human Lipopolysaccharides, LPS ELISA Kit" CSB-E09945h.
It is used to measure circulating levels of Gut-Derived Serum Lipopolysaccharide?
How many blood samples or individuals can be analyzed with the kit?

Thanks for your inquiry.
For a 96T kit, it can test 88 samples. If you run dupliacated wells, it can test 44 samples.
Pls follow actual test result if you want to test Gut-Derived Serum Lipopolysaccharide.Pls let me know if you have any further questions. Thank you.

I'm wondering why there are different ELISA kits against different species. Is it because of the variation of LPS? Can I use the human LPS ELISA to measure the LPS in mouse plasma?


Human LPS ELISA kit is used to test human samples, while mouse LPS ELISA kit is used to test mouse samples. Detection of cross-species is not advised. Because different species are different in the matrix.

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