Human Lipopolysaccharides,LPS ELISA Kit

Code CSB-E09945h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation LPS
Target Name Lipopolysaccharides,LPS
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 92  
Range % 87-96  
1:8 Average % 94  
Range % 89-99  
Recovery
The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-97  
EDTA plasma (n=4) 88 82-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.809 2.960 2.885 2.720  
200 2.227 2.366 2.297 2.132  
100 1.515 1.556 1.536 1.371  
50 0.789 0.755 0.772 0.607  
25 0.432 0.470 0.451 0.286  
12.5 0.303 0.325 0.314 0.149  
6.25 0.235 0.232 0.234 0.069  
0 0.164 0.165 0.165    
References
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Q&A and Customer Reviews

Q&A
Q:

I would like to know if this ELISA kit is SPECIFIC for LPS of Escherichia Coli
I mean CSB-E09945h ELISA kit

A:
Thanks for your inquiry.
CSB-E09945h detects the residual LPs in the human body due to infection.Pls let me know if you have any further questions. Thank you.
Q:

Could you share with me what the antibodies in this kit bind to CSB-E09945h?

A:
Thanks for your inquiry.
CSB-E09945h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.Pls let me know if you have any further questions. Thank you.
Q:

I have an interest in knowing product information "Human Lipopolysaccharides, LPS ELISA Kit" CSB-E09945h.
It is used to measure circulating levels of Gut-Derived Serum Lipopolysaccharide?
How many blood samples or individuals can be analyzed with the kit?

A:
Thanks for your inquiry.
CSB-E09945h
For a 96T kit, it can test 88 samples. If you run dupliacated wells, it can test 44 samples.
Pls follow actual test result if you want to test Gut-Derived Serum Lipopolysaccharide.Pls let me know if you have any further questions. Thank you.
Customer Reviews

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