Human Lipopolysaccharides,LPS ELISA Kit

Instructions
Code CSB-E09945h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The human lipopolysaccharides/LPS ELISA kit is a ready-to-use microwell, strip plate ELISA kit for the quantitative measurement of human LPS in biological samples, including serum, plasma, and tissue homogenates. It employs the sandwich ELISA technique in the detection of target analytes in the samples. This ELISA kit has been quality controlled in multiple characteristics, including sensitivity, specificity, precision, linearity, and recovery. See the product instructions to obtain more information.

LPS, a major component of Gram-negative bacteria, is a pathogen-associated molecular pattern (PAMP) molecule that mediates the bacterial infection. It also plays an important role in endotoxic shock. LPS-TLR4-MD2 interaction activates the MYD88-dependent pathway that leads to inflammation and bacterial clearance and the MYD88-independent pathway critical for adjuvanticity.

Target Name Lipopolysaccharides,LPS
Abbreviation LPS
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-94  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 92  
Range % 87-96  
1:8 Average % 94  
Range % 89-99  
Recovery
The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-97  
EDTA plasma (n=4) 88 82-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.809 2.960 2.885 2.720  
200 2.227 2.366 2.297 2.132  
100 1.515 1.556 1.536 1.371  
50 0.789 0.755 0.772 0.607  
25 0.432 0.470 0.451 0.286  
12.5 0.303 0.325 0.314 0.149  
6.25 0.235 0.232 0.234 0.069  
0 0.164 0.165 0.165    
Materials provided
  • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
  • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
  • Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
  • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
  • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
  • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
  • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
  • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
  • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

I would like to know if this ELISA kit is SPECIFIC for LPS of Escherichia Coli
I mean CSB-E09945h ELISA kit

A:
Thanks for your inquiry.
CSB-E09945h detects the residual LPs in the human body due to infection.Pls let me know if you have any further questions. Thank you.
Q:

Could you share with me what the antibodies in this kit bind to CSB-E09945h?

A:
Thanks for your inquiry.
CSB-E09945h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.Pls let me know if you have any further questions. Thank you.
Q:

I have an interest in knowing product information "Human Lipopolysaccharides, LPS ELISA Kit" CSB-E09945h.
It is used to measure circulating levels of Gut-Derived Serum Lipopolysaccharide?
How many blood samples or individuals can be analyzed with the kit?

A:
Thanks for your inquiry.
CSB-E09945h
For a 96T kit, it can test 88 samples. If you run dupliacated wells, it can test 44 samples.
Pls follow actual test result if you want to test Gut-Derived Serum Lipopolysaccharide.Pls let me know if you have any further questions. Thank you.

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  Email: support@cusabio.com
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