Rat Mast Cell Tryptase,MCT ELISA Kit

Code CSB-E13627r
Size 96T,5×96T,10×96T
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Product Details

Target Name
tryptase alpha/beta 1
Alternative Names
Tpsab1 ELISA Kit; Mcp7 ELISA Kit; Mcpt7 ELISA Kit; Tpsb1 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; rMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit; Tryptase ELISA Kit; skin ELISA Kit
Abbreviation
TPSAB1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, urine
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 90-92  
1:2 Average % 92  
Range % 87-96  
1:4 Average % 94  
Range % 89-97  
1:8 Average % 87  
Range % 82-92  
Recovery
The recovery of rat MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-102  
EDTA plasma (n=4) 104 100-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.464 2.489 2.477 2.326  
20 1.823 1.863 1.843 1.692  
10 1.229 1.263 1.246 1.095  
5 0.812 0.847 0.830 0.679  
2.5 0.482 0.498 0.490 0.339  
1.25 0.312 0.339 0.326 0.175  
0.625 0.241 0.258 0.250 0.099  
0 0.150 0.152 0.151    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TPSAB1 ELISA Kit was designed for the quantitative measurement of Rat TPSAB1 protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
Subcellular Location
Secreted. Note=Released from the secretory granules upon mast cell activation.
Protein Families
Peptidase S1 family, Tryptase subfamily
Tissue Specificity
Mast cells.
Database Links
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