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Recombinant Rat Tryptase (Tpsab1)
In StockCode CSB-EP024127RA Abbreviation Recombinant Rat Tpsab1 protein MSDS Size US$306 Order now Image -
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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PurityGreater than 90% as determined by SDS-PAGE.Target NamesTpsab1Uniprot No.Research AreaImmunologyAlternative NamesTpsab1; Mcp7; Mcpt7; Tpsb1; Tryptase; EC 3.4.21.59; Mast cell protease 7; rMCP-7; Tryptase alpha/beta-1; Tryptase; skinSpeciesRattus norvegicus (Rat)SourceE.coliExpression Region29-273aaTarget Protein SequenceIVGGQEASGNKWPWQVSLRVNDTYWMHFCGGSLIHPQWVLTAAHCVGPNKADPNKLRVQLRKQYLYYHDHLLTVSQIISHPDFYIAQDGADIALLKLTNPVNITSNVHTVSLPPASETFPSGTLCWVTGWGNINNDVSLPPPFPLEEVQVPIVENRLCDLKYHKGLNTGDNVHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIYRYVPKYF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.Mol. Weight47.4kDaProtein LengthFull Length of Mature ProteinTag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
FormLiquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.Troubleshooting and FAQsStorage ConditionStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.Shelf LifeThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Lead Time3-7 business daysNotesRepeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.Datasheet & COAPlease contact us to get it.Related Products
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Target Background
FunctionTryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.Subcellular LocationSecreted. Note=Released from the secretory granules upon mast cell activation.Protein FamiliesPeptidase S1 family, Tryptase subfamilyTissue SpecificityMast cells.Database Links -
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