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Rat Myeloperoxidase (MPO) ELISA kit

Citations (26) Protocol MSDS
Code CSB-E08722r
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
myeloperoxidase,MPO
Abbreviation
MPO
Uniprot No.
D3ZGE2
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %91
Range %86-95
1:2Average %94
Range %90-98
1:4Average %91
Range %85-97
1:8Average %97
Range %91-102
Recovery
The recovery of rat MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9287-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.347 2.232 2.290 2.109
101.416 1.386 1.401 1.220
50.902 0.887 0.895 0.714
2.50.602 0.612 0.607 0.426
1.250.421 0.439 0.430 0.249
0.6250.312 0.326 0.319 0.138
0.3120.274 0.256 0.265 0.084
00.182 0.180 0.181 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat MPO ELISA Kit was designed for the quantitative measurement of Rat MPO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

Citations

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Tissue homogenate Lung

Sample species: Rat

Sample dilution: No dilution

Review: I used CSB-E08224r to detect the content of HMGB1 in the homogenate of rat colon lung tissue. The product is convenient and fast, with high sensitivity, reliable experimental results, obvious trends, and it is recommended to use. Rat colon tissue, the sample concentration range is 10ng/mL-16ng/mL. The previous 6 boxes were rat samples, and the total sample size is sufficient, so no dilution was performed.

By Anonymous

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