Rat Neuron-specific enolase,NSE ELISA Kit

Code CSB-E07963r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat ENO2 ELISA Kit was designed for the quantitative measurement of Rat ENO2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

Target Name enolase 2 (gamma, neuronal)
Alternative Names Eno2 ELISA Kit; Eno-2 ELISA Kit; Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
Abbreviation ENO2
Uniprot No. P07323
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %85-94
1:2Average %100
Range %97-104
1:4Average %90
Range %87-94
1:8Average %100
Range %95-103
Recovery
The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-98
EDTA plasma (n=4)9592-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.260 2.232 2.246 2.078
201.454 1.486 1.470 1.302
100.920 0.928 0.924 0.756
50.654 0.663 0.659 0.491
2.50.403 0.398 0.401 0.233
1.250.322 0.314 0.318 0.150
0.6250.266 0.257 0.262 0.094
00.170 0.166 0.168  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Subcellular Location Cytoplasm. Cell membrane.
Protein Families Enolase family
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Database Links

KEGG: rno:100911625

STRING: 10116.ENSRNOP00000005601

UniGene: Rn.10828

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