Rat Neuron-specific enolase,NSE ELISA Kit

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Rat Neuron-specific enolase,NSE ELISA Kit

Name: Rat Neuron-specific enolase,NSE ELISA Kit
Brand: CUSABIO
SKU: CSB-E07963r

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Code	CSB-E07963r
Size	96T,5×96T,10×96T
Trial Size
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Target Background

Target Name

enolase 2 (gamma, neuronal)

Alternative Names

Eno2 ELISA Kit; Eno-2 ELISA Kit; Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit

Abbreviation

ENO2

Uniprot No.

P07323

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	100
	Range %	97-104
1:4	Average %	90
	Range %	87-94
1:8	Average %	100
	Range %	95-103

Recovery

The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	95	92-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.260	2.232	2.246	2.078
20	1.454	1.486	1.470	1.302
10	0.920	0.928	0.924	0.756
5	0.654	0.663	0.659	0.491
2.5	0.403	0.398	0.401	0.233
1.25	0.322	0.314	0.318	0.150
0.625	0.266	0.257	0.262	0.094
0	0.170	0.166	0.168

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Reversing Postcardiopulmonary Bypass Associated Cognitive Dysfunction Using k-Opioid Receptor Agonists to Regulate Microglial Polarization via the NLRP3/Caspase-1 Pathway Pei Song,Journal of Healthcare Engineering,2021
Magnetic resonance spectroscopy for assessment of brain injury in the rat model ofSepsis Miaoyun Wen.et al,Experimental and Therapeutic Medicine,2017
Dexmedetomidine reduces the neuronal apoptosis related to cardiopulmonary bypass by inhibiting activation of the JAK2-STAT3 pathway Chen Y.et al,Drug Des Devel Ther,2017
Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury andAugments inflammatory response in rats. Zhu SZ.et al,Biochem Biophys Res Commun.,2016
Endotoxin-induced lung alveolar cell injury causes brain cell damage Rodrguez-Gonzlez R.et al,Exp Biol Med (Maywood).,2015
Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model Saha L et al,Pharmacol Biochem Behav.,2014
Interrupted intracarotid artery cold saline infusion as an alternative method for neuroprotection after ischemic stroke Ya-Bin Ji et al,Neurosurgical Focus,2012
Therapeutic time window of hypothermia is broader than cerebral artery flushing in carotid saline infusion after transient focal ischemic stroke in rats /,Neurological Research,,2012

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Function (From Uniprot)	Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Subcellular Location	Cytoplasm. Cell membrane.
Protein Families	Enolase family
Tissue Specificity	The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Database Links	KEGG: rno:100911625 STRING: 10116.ENSRNOP00000005601 UniGene: Rn.10828

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HIF-1 signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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