Rat Pro-opiomelanocortin(POMC) ELISA KIT

Code CSB-E13569r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
proopiomelanocortin
Alternative Names
Pomc ELISA kit; Pomc2Pro-opiomelanocortin ELISA kit; POMC ELISA kit; Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma ELISA kit; Gamma-MSH); Potential peptide; Corticotropin ELISA kit; Adrenocorticotropic hormone ELISA kit; ACTH); Melanocyte-stimulating hormone alpha ELISA kit; Alpha-MSH ELISA kit; Melanotropin alpha); Corticotropin-like intermediary peptide ELISA kit; CLIP); Lipotropin beta ELISA kit; Beta-LPH); Lipotropin gamma ELISA kit; Gamma-LPH); Melanocyte-stimulating hormone beta ELISA kit; Beta-MSH ELISA kit; Melanotropin beta); Beta-endorphin; Met-enkephalin] ELISA kit
Abbreviation
POMC
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 99  
Range % 93-105  
1:10 Average % 93  
Range % 90-97  
1:20 Average % 95  
Range % 90-100  
1:40 Average % 84  
Range % 80-88  
Recovery
The recovery of rat POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-95  
EDTA plasma (n=4) 87 85-90  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.502 2.603 2.553 2.438  
250 1.661 1.762 1.712 1.597  
125 1.127 1.148 1.138 1.023  
62.5 0.724 0.713 0.719 0.604  
31.2 0.388 0.377 0.383 0.268  
15.6 0.287 0.276 0.282 0.167  
7.8 0.184 0.181 0.183 0.068  
0 0.115 0.114 0.115    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat POMC ELISA Kit was designed for the quantitative measurement of Rat POMC protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Stimulates the adrenal glands to release cortisol.; Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.; Increases the pigmentation of skin by increasing melanin production in melanocytes.; Endogenous orexigenic opiate.; Endogenous opiate.
Subcellular Location
Secreted.
Protein Families
POMC family
Tissue Specificity
ACTH and MSH are produced by the pituitary gland.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1