Rat Protein S100-A1(S100A1) ELISA kit

Instructions
Code CSB-EL020622RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name S100 calcium binding protein A1
Alternative Names S100a1 ELISA Kit; Protein S100-A1 ELISA Kit; S-100 protein alpha chain ELISA Kit; S-100 protein subunit alpha ELISA Kit; S100 calcium-binding protein A1 ELISA Kit
Abbreviation S100A1
Uniprot No. P35467
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-97
1:2 Average % 87
Range % 82-91
1:4 Average % 98
Range % 94-102
1:8 Average % 101
Range % 95-107
Recovery
The recovery of rat S100A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 84 80-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
400 2.043 2.001 2.022 1.851
200 1.295 1.327 1.311 1.140
100 0.888 0.852 0.870 0.699
50 0.608 0.640 0.624 0.453
25 0.430 0.460 0.445 0.274
12.5 0.346 0.329 0.338 0.167
6.25 0.255 0.238 0.247 0.076
0 0.168 0.174 0.171  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Probably acts as a Ca(2+) signal transducer (By similarity). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers a conformational change
Gene References into Functions
  1. depletion of S100A1 was observed in ischemic cardiomyocytes, and S100A1 plasma concentration was also significantly higher than that of the sham-operated group PMID: 23683996
  2. S100A1 is involved in the maintenance of the genetic program that defines normal myocardial function and its downregulation is permissive for the induction of genes that underlie myocardial hypertrophy PMID: 12619862
  3. S100A1 and calmodulin compete for the same binding site on ryanodine receptor PMID: 18650434
  4. in sympathetic neurons exogenous S100A1 may lead to an increase of sympathetic output. PMID: 19657060
Subcellular Location Cytoplasm
Protein Families S-100 family
Tissue Specificity Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Database Links

KEGG: rno:295214

STRING: 10116.ENSRNOP00000017000

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