Rat Rheumatoid Factor(RF) ELISA Kit

Code CSB-E13666r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Rheumatoid Factor(RF)
Alternative Names N/A
Abbreviation RF
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 15.6 mIU/mL-1000 mIU/mL
Sensitivity 3.9 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:5Average %104
Range %96-108
1:10Average %94
Range %91-98
1:20Average %102
Range %98-106
1:40Average %88
Range %82-96
The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-95
EDTA plasma (n=4)9992-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.646 2.545 2.596 2.443
5001.975 2.076 2.026 1.873
2501.284 1.308 1.296 1.143
1250.751 0.762 0.757 0.604
62.50.512 0.501 0.507 0.354
31.20.353 0.342 0.348 0.195
15.60.285 0.281 0.283 0.130
00.153 0.152 0.153  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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