Rat Rheumatoid Factor(RF) ELISA Kit

Code CSB-E13666r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Rheumatoid Factor(RF)
Alternative Names
N/A
Abbreviation
RF
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
15.6 mIU/mL-1000 mIU/mL
Sensitivity
3.9 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %104
Range %96-108
1:10Average %94
Range %91-98
1:20Average %102
Range %98-106
1:40Average %88
Range %82-96
Recovery
The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-95
EDTA plasma (n=4)9992-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
10002.646 2.545 2.596 2.443
5001.975 2.076 2.026 1.873
2501.284 1.308 1.296 1.143
1250.751 0.762 0.757 0.604
62.50.512 0.501 0.507 0.354
31.20.353 0.342 0.348 0.195
15.60.285 0.281 0.283 0.130
00.153 0.152 0.153  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat Rheumatoid Factor (RF) ELISA Kit was designed for the quantitative measurement of Rat Rheumatoid Factor (RF) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 mIU/mL-1000 mIU/mL and the sensitivity is 3.9 mIU/mL .

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X