Rat Rheumatoid Factor(RF) ELISA Kit

Instructions
Code CSB-E13666r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name Rheumatoid Factor(RF)
Alternative Names N/A
Abbreviation RF
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 15.6 mIU/mL-1000 mIU/mL
Sensitivity 3.9 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %104
Range %96-108
1:10Average %94
Range %91-98
1:20Average %102
Range %98-106
1:40Average %88
Range %82-96
Recovery
The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-95
EDTA plasma (n=4)9992-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
10002.646 2.545 2.596 2.443
5001.975 2.076 2.026 1.873
2501.284 1.308 1.296 1.143
1250.751 0.762 0.757 0.604
62.50.512 0.501 0.507 0.354
31.20.353 0.342 0.348 0.195
15.60.285 0.281 0.283 0.130
00.153 0.152 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

  Tel: 301-363-4651
  Email: support@cusabio.com
  Distributors Worldwide

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1