Rat Runt-related transcription factor 2(RUNX2) ELISA kit

Instructions
Code CSB-EL020594RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name runt-related transcription factor 2
Alternative Names Runx2 ELISA Kit; Cbfa1 ELISA Kit; Osf2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; CBF-alpha-1 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OSF-2 ELISA Kit; Fragments ELISA Kit
Abbreviation RUNX2
Uniprot No. Q9Z2J9
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat RUNX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 91-103  
1:2 Average % 102  
Range % 94-107  
1:4 Average % 88  
Range % 83-91  
1:8 Average % 94  
Range % 84-98  
Recovery
The recovery of rat RUNX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 103 93-107  
EDTA plasma (n=4) 98 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
200 2.551 2.452 2.502 2.322  
100 2.126 1.996 2.061 1.881  
50 1.359 1.341 1.350 1.170  
25 0.793 0.818 0.806 0.626  
12.5 0.474 0.481 0.478 0.298  
6.25 0.395 0.403 0.399 0.219  
3.12 0.266 0.257 0.262 0.082  
0 0.182 0.178 0.180    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation
Subcellular Location Nucleus
Database Links

STRING: 10116.ENSRNOP00000057733

UniGene: Rn.83672

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