Rat Serine/threonine-protein kinase TNNI3K(TNNI3K) ELISA kit

Instructions
Code CSB-EL024014RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name TNNI3 interacting kinase
Alternative Names Tnni3k ELISA Kit; Cark ELISA Kit; Serine/threonine-protein kinase TNNI3K ELISA Kit; EC 2.7.11.1 ELISA Kit; Cardiac ankyrin repeat kinase ELISA Kit; TNNI3-interacting kinase ELISA Kit
Abbreviation TNNI3K
Uniprot No. Q7TQP6
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.69 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 86-95
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rat TNNI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1200 2.465 2.561 2.513 2.401
600 1.932 1.892 1.912 1.800
300 1.277 1.298 1.288 1.176
150 0.708 0.739 0.724 0.612
75 0.412 0.432 0.422 0.310
37.5 0.287 0.275 0.281 0.169
18.75 0.183 0.185 0.184 0.072
0 0.111 0.113 0.112  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function May play a role in cardiac physiology.
Gene References into Functions
  1. TNNI3K may be a novel mediator of cardiac troponin I phosphorylation and contribute to the regulation of cardiac myofilament contraction function. PMID: 23369981
  2. TNNI3K promotes a concentric hypertrophy with enhancement of cardiac function via regulating the phosphorylation of cTnI PMID: 23472207
Subcellular Location Nucleus, Cytoplasm
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
Database Links

KEGG: rno:295531

STRING: 10116.ENSRNOP00000031479

UniGene: Rn.3434

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