Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1(sLOX-1)ELISA Kit

Code CSB-E15845r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat OLR1 ELISA Kit was designed for the quantitative measurement of Rat OLR1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name oxidized low density lipoprotein (lectin-like) receptor 1
Alternative Names Olr1; Lox1; Oldlr1; Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1
Abbreviation OLR1
Uniprot No. O70156
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sLOX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-94
1:2Average %92
Range %88-99
1:4Average %88
Range %84-92
1:8Average %95
Range %90-100
Recovery
The recovery of rat sLOX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8580-90
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2AverageCorrected
102.375 2.227 2.301 2.190
51.816 1.902 1.859 1.748
2.51.240 1.208 1.224 1.113
1.250.725 0.785 0.755 0.644
0.6250.421 0.439 0.430 0.319
0.3120.263 0.284 0.274 0.163
0.1560.218 0.221 0.220 0.109
00.109 0.112 0.111
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
Gene References into Functions
  1. an important role of LOX-1 in the maintenance of PASMCs phenotype. PMID: 29069578
  2. data support an important role for STBEVs in impairment of vascular function via activation of LOX-1 and reduced nitric oxide mediated vasodilation. Moreover, we postulate that the LOX-1 pathway could be a potential therapeutic target in pathologies associated with vascular dysfunction during pregnancy PMID: 28672042
  3. Negative feedback regulation between let-7g and LOX-1 mediated hypoxia-induced proliferation of in pulmonary artery smooth muscle cells (PASMCs). PMID: 28108289
  4. LOX-1 promotes right ventricular hypertrophy in hypoxia-exposed rats PMID: 28259654
  5. Losartan attenuated lung injury by suppressing LOX-1 expression to alleviate inflammation and cell apoptosis. PMID: 26884836
  6. Swimming exercise reduced heart expression of LOX-1 receptor and free radical production in experimental diabetes. PMID: 26432573
  7. OLR1 is a novel molecular link between the proliferative and inflammatory responses of vascular smooth muscle cells. PMID: 26305474
  8. data indicated that olmesartan may attenuate the impairment of endothelial cell via down-regulation of the increased LOX-1 expression induced by ox-LDL. PMID: 22408405
  9. EETs exert beneficial effects on ox-LDL-induced inflammation primarily through the inhibition of LOX-1 receptor upregulation, MAPK phosphorylation, and NF-kappaB activation and through the upregulation of CYP2J4 expression. PMID: 24486707
  10. LOX-1 is a promising therapeutic target in neonatal HIE, and the inhibition of LOX-1 may become a novel treatment for babies who have experienced asphyxia. PMID: 24731447
  11. results indicate that LOX-1 is essential in microglia for promoting an inflammatory response in the presence of soluble neuronal-injury signals such as extracellular HSP60, thereby linking neuroinflammation and neurotoxicity PMID: 22847064
  12. LOX-1 activation by oxLDL and subsequent peroxynitrite generation as a novel mechanism by which disruption of the BBB occurs in EPE. PMID: 23230281
  13. These findings indicated that LOX-1 expression was up-regulated in the cortex of SHR and its expression has implication in neuronal apoptosis. PMID: 22885180
  14. In a rat model of preeclampsia, there is dramatic increase in expression levels of both LOX-1 receptor and endothelial NO synthase enzyme, along with increased superoxide production and modestly decreased endothelial function. PMID: 22392901
  15. Inhibition of rat LOX-1 receptor reduces leukocyte adhesion in experimental endotoxemia. PMID: 21143965
  16. Data show that a high fat diet elevates LOX-1 protein and mRNA expression in aorta, and that sleeve gastrectomy decreases plasma LDL levels, and downregulates LOX-1 protein and mRNA expression. PMID: 21990956
  17. LOX-1 appears to be involved in CRP-induced complement activation, and thus may serve to locate the site of CRP-induced complement activation and inflammation. PMID: 21821723
  18. enhanced expression and activation of LOX-1 mediated the enhancement of vascular permeability, which contributed to the vascular lipid accumulation under hypertensive states PMID: 20216085
  19. expression pattern of LOX1-R in brain microvessels endothelial cells and its modulation by astrocytes PMID: 12095612
  20. findings indicate that myocardial ischemia-reperfusion upregulates LOX-1 expression, which through p38 MAPK activation increases the expression of MMP-1 and adhesion molecules PMID: 12384456
  21. LOX-1 may have a role in the pathogenesis of osteoarthritis, inducing chondrocyte death through PI3 kinase/Akt pathway. PMID: 12435393
  22. LOX-1 is an adhesion molecule involved in leukocyte recruitment and may represent an attractive target for modulation of endotoxin-induced inflammation PMID: 12538855
  23. enhanced renal expression of olr1 might play some roles in the progression of chronic renal failure in rats. PMID: 12661921
  24. LOX-1 up-regulation is redox-sensitive PMID: 16055083
  25. the bradykinin-eNOS and oxidative stress-LOX-1 pathway have roles in cardiovascular remodeling under chronic angiotensin-converting enzyme inhibition PMID: 16214149
  26. LOX-1 activation driven by lipid oxidants in the preurine fluid is critical in inflammatory changes PMID: 18322020
  27. Oxidized low-density lipoprotein induces matrix metalloproteinase-9 expression at transcriptional and translational level in brain astrocytes, which are mediated through mitogen-activated protein kinase signaling pathways leading to AP-1 activation. PMID: 18661553

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Subcellular Location Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted.
Tissue Specificity Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.
Database Links

KEGG: rno:140914

STRING: 10116.ENSRNOP00000011196

UniGene: Rn.87449

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