Rat Steroid 5-Alpha-Reductase 1 (SRD5A1) ELISA Kit

Code CSB-E14053r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
Alternative Names Srd5a1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
Abbreviation SRD5A1
Uniprot No. P24008
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:5Average %100
Range %95-105
1:10Average %96
Range %90-102
1:20Average %94
Range %88-99
1:40Average %90
Range %85-95
The recovery of rat SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10198-105
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.762 2.803 2.783 2.589
5002.117 2.104 2.111 1.917
2501.598 1.583 1.591 1.397
1251.103 1.136 1.120 0.926
62.50.652 0.672 0.662 0.468
31.250.416 0.426 0.421 0.227
15.60.341 0.351 0.346 0.152
00.193 0.195 0.194  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Gene References into Functions
  1. Ziram inhibited SRD5A1 and AKR1C14 activities, thus possibly interfering with neurosteroid production in rats. PMID: 28707553
  2. Exposure of middle-aged rats to environmental enrichment increased mRNA levels of 5al;pha-reductase-1, 3alpha-HSD and cytochrome P450 17alpha-hydroxylase/c17,20-lyase and decreased the methylation state of the 5alpha-reductase-1 gene PMID: 26021641
  3. Low Srd5a1 expression is associated with benign prostatic hypertrophy. PMID: 23405234
  4. Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress. PMID: 23122426
  5. Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood PMID: 22131296
  6. Elevated levels of 5alpha-reduced androgens seen in hyperinsulinemic conditions might be explained on the basis of a stimulatory effect of insulin on 5alpha-reductase in granulosa cells. PMID: 20810561
  7. Thus, the present results indicate that the vitamin E-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation. PMID: 20447326
  8. 5 alpha-R1 immunostaining was detected mainly in the white matter (Wm). PMID: 15305365
  9. great sexual dimorphism in the central nervous system (CNS) with respect to both 5alpha-reductase isozymes, and suggest a crucial role of dihydrotestosterone in the sexual dimorphism of the CNS in the female PMID: 16595696
  10. Our results demonstrate that both 5alpha-R isozymes are significantly higher in prefrontal cortex of male rats after acute swim stress in comparison with control rats PMID: 17826869
  11. Our results suggest that 5AR inhibitors elicit antipsychotic-like effects in animals and may be proposed as a putative novel target in the management of psychotic disorders. PMID: 18354385

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Subcellular Location Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families Steroid 5-alpha reductase family
Tissue Specificity Liver and prostate (at a low level).
Database Links

KEGG: rno:24950

STRING: 10116.ENSRNOP00000023659

UniGene: Rn.4620

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