Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

Code CSB-E08729r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Stromal cell derived factor 1a,SDF-1a
Abbreviation
SDF1a/CXCL12
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.78 pg/mL-50 pg/mL
Sensitivity
0.195 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 88  
Range % 83-97  
1:200 Average % 97  
Range % 91-103  
1:400 Average % 84  
Range % 80-88  
1:800 Average % 89  
Range % 81-95  
Recovery
The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-96  
EDTA plasma (n=4) 97 94-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
50 2.830 2.775 2.803 2.666  
25 2.326 2.453 2.390 2.253  
12.5 1.600 1.632 1.616 1.479  
6.25 0.937 0.894 0.916 0.779  
3.12 0.528 0.532 0.530 0.393  
1.56 0.321 0.335 0.328 0.191  
0.78 0.157 0.148 0.153 0.016  
0 0.140 0.133 0.137    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Stromal cell-derived factor 1a (SDF-1a) ELISA Kit is a reliable and efficient solution for researchers in the field of immunology. This ELISA kit enables the quantitative measurement of SDF-1a in a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates from Rattus norvegicus (Rat).

With a detection range of 0.78 pg/mL to 50 pg/mL and a sensitivity of 0.195 pg/mL, this ELISA kit delivers accurate and precise results, making it a valuable tool for researchers working on SDF-1a in rats. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

This ELISA kit works on a sandwich assay principle, where the SDF-1a antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

The Rat SDF-1a ELISA Kit has been cited in more than 4 publications, highlighting its reliability and utility in research. It is an excellent addition to any immunology research laboratory, providing accurate and precise measurement of SDF-1a in rat samples.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1