Recombinant Rat Chemokine (C-X-C motif) ligand 12 (Stromal cell-derived factor 1) protein (Cxcl12) (Active)

CXCL12/SDF-1 is a key ligand for CXCR4 and plays a central role in leukocyte trafficking, hematopoietic stem cell homing, and tumor metastasis, making precise functional reagents essential for mechanistic studies. This tag-free recombinant rat CXCL12 encompasses the full-length mature sequence (residues 22–89) expressed in *E. coli*, and it demonstrates confirmed chemotactic activity on human peripheral blood monocytes at 100–200 ng/ml, providing a suitable basis for Transwell migration assays, CXCR4 signaling pathway analyses involving MAPK and PI3K/Akt cascades, and in vivo rat models of inflammation or tumor biology. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound monocyte and lymphocyte functional readouts, which supports use in sensitive cell-based activation, proliferation, and differentiation experiments. Purity exceeding 97% by SDS-PAGE further aligns with standards expected in antibody validation workflows, ELISA coating, and reference standard applications for cytokine detection assays.