Rat Syndecan-2(SDC2) ELISA kit

Code CSB-EL020889RA
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
syndecan 2
Alternative Names
Sdc2; Hspg1; Synd2; Syndecan-2; SYND2; Fibroglycan; Heparan sulfate proteoglycan core protein; HSPG; CD antigen CD362
Abbreviation
SDC2
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %99
Range %93-105
1:2Average %96
Range %91-100
1:4Average %94
Range %89-98
1:8Average %85
Range %81-89
Recovery
The recovery of rat SDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9690-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.562 2.463 2.513 2.388
1002.113 1.994 2.054 1.929
501.520 1.491 1.506 1.381
251.053 1.044 1.049 0.924
12.50.614 0.623 0.619 0.494
6.250.361 0.370 0.366 0.241
3.120.239 0.243 0.241 0.116
00.124 0.125 0.125  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat SDC2 ELISA Kit was designed for the quantitative measurement of Rat SDC2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis.
Gene References into Functions
  1. postsynaptic SDC2 uses its ectodomain to interact with and facilitate dendritic filopodial targeting of FGF22, triggering presynaptic maturation. PMID: 27627962
  2. Data suggest that the cytoplasmic domain of Sdc2 is involved in regulation of expression of MMP7 in colon carcinoma/adenocarcinoma cells; induction of MMP7 involves protein kinase C gamma-mediated FAK/ERK signaling. (Sdc2 = syndecan-2; MMP7 = matrix metalloproteinase-7; FAK = focal adhesion kinase 1) PMID: 28821612
  3. Syndecan-2 and -4 form heterdimer and this interaction regulates syndecan-mediated cellular functions. PMID: 25979339
  4. Data suggest that phenylalanine Phe(167) in the transmembrane domain of syndecan-2 endows the protein with specific functions. PMID: 25572401
  5. Data suggest that Y-P30 can control the nuclear localization of Calcium/calmodulin-dependent serine kinase (CASK) in a syndecan (SDC) -2 and -3 -dependent manner. PMID: 24498267
  6. syndecan-2 expression occurs in developmentally regulated patterns and syndecan-2 probably has different roles in adult and developing anterior pituitary glands. PMID: 23673414
  7. pleiotrophin (PTN) and syndecan-2 and -3 as direct binding partners of Y-P30. PMID: 18599487
  8. findings suggest that Sdc-2 plays an important role in microglial production of inflammatory cytokines, chemokines, and reactive oxygen species in hypoxic conditions PMID: 18803305
  9. these results demonstrate a novel cooperation between syndecan-2 and integrin alpha2beta1 in adhesion-mediated cell migration and invasion. PMID: 19394307
  10. Syndecan-2 plays a crucial role in the migratory potential of melanoma cells. PMID: 19641225

Show More

Hide All

Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Syndecan proteoglycan family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1