Rat Tenascin-C(TN-C) ELISA Kit

Code CSB-E13377r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Tenascin-C(TN-C)
Abbreviation
TN-C
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
2.5 ng/mL-160 ng/mL
Sensitivity
0.625 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 88-96    
1:2 Average % 84    
Range % 80-88    
1:4 Average % 106    
Range % 101-113    
1:8 Average % 93    
Range % 89-98    
Recovery
The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 88 84-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
160 2.417 2.502 2.460 2.301    
80 2.102 2.173 2.138 1.979    
40 1.700 1.615 1.658 1.499    
20 1.085 1.049 1.067 0.908    
10 0.671 0.684 0.678 0.519    
5 0.424 0.437 0.431 0.272    
2.5 0.293 0.281 0.287 0.128    
0 0.162 0.156 0.159      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TN-C ELISA Kit was designed for the quantitative measurement of Rat TN-C protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.5 ng/mL-160 ng/mL and the sensitivity is 0.625 ng/mL.

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