Rat Thyroglobulin,TG ELISA Kit

Code CSB-E08240r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P06882
Lead Time 3-5 working days
Abbreviation TG
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name thyroglobulin
Alias AITD3, TGN,
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Thyroid hormones biosynthesis
Sample Types serum, plasma, tissue Homogenates
Detection Range 10 ng/mL-160 ng/mL
Sensitivity 10 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.
HGNC 20606
RGD 1309717
MGI 98715
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 89-102
1:2 Average % 92
Range % 86-99
1:4 Average % 95
Range % 87-105
1:8 Average % 90
Range % 83-102
Recovery
The recovery of rat TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 89-100
EDTA plasma (n=4) 95 88-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
10 2.553 2.568 2.561
20 1.748 1.763 1.756
40 0.885 0.875 0.880
80 0.515 0.511 0.513
160 0.285 0.287 0.286
References
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Function Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Involvement in disease Defects in Tg are a cause of a form of hypothyroidism in rdw rat.
Subcellular Location Secreted
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Thyroid gland specific.
Database Links

STRING: 10116.ENSRNOP00000009241

UniGene: Rn.10429

Pathway Th17 cell differentiation
Thyroid hormone synthesis

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