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Rat Thyroglobulin,TG ELISA Kit

Citations (7) Protocol
Code CSB-E08240r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
thyroglobulin
Alternative Names
TgThyroglobulin ELISA kit; Tg ELISA kit
Abbreviation
TG
Uniprot No.
P06882
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue Homogenates
Detection Range
10 ng/mL-160 ng/mL
Sensitivity
10 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 95
Range % 89-102
1:2 Average % 92
Range % 86-99
1:4 Average % 95
Range % 87-105
1:8 Average % 90
Range % 83-102
Recovery
The recovery of rat TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 89-100
EDTA plasma (n=4) 95 88-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
10 2.553 2.568 2.561
20 1.748 1.763 1.756
40 0.885 0.875 0.880
80 0.515 0.511 0.513
160 0.285 0.287 0.286
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Thyroglobulin (TG) ELISA Kit is a perfect solution for researchers studying metabolism，thyroid-related diseases. This quantitative assay kit allows for the detection of Thyroglobulin (Tg) in serum, plasma, and tissue homogenates of Rattus norvegicus (rat) samples.

With a detection range of 10 ng/mL to 160 ng/mL and a sensitivity of 10 ng/mL, this ELISA kit provides accurate and reliable results for your research needs. The assay time is quick, ranging from 1-5 hours, and the sample volume required is only 50-100 ul.

The detection wavelength for this kit is 450 nm, providing high sensitivity and specificity for the measurement of Tg in rat samples. The assay principle is based on the competitive method, providing high specificity and accuracy for the detection of this protein.

This Rat Thyroglobulin (TG) ELISA Kit has been cited in over 5 peer-reviewed publications, demonstrating the reliability and accuracy of this kit in the scientific community.

Citations

Related Products

Tg Antibodies

Tg Proteins

See all products about Tg >>

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Target Background

Function
(From Uniprot)
Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.
Involvement in disease
Defects in Tg are a cause of a form of hypothyroidism in rdw rat.
Subcellular Location
Secreted.
Protein Families
Type-B carboxylesterase/lipase family
Tissue Specificity
Specifically expressed in the thyroid gland.
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Related pathways
Th17 cell differentiation Thyroid hormone synthesis
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