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Rat Thyroglobulin,TG ELISA Kit

Rat Thyroglobulin,TG ELISA Kit

Specification
Target

Code

CSB-E08240r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P06882

Lead Time

3-5 working days

Abbreviation

Protein Biological Process 1

Biosynthesis/Metabolism

Target Name

thyroglobulin

Alias

AITD3, TGN,

Species

Rattus norvegicus (Rat)

Protein Biological Process 3

Thyroid hormones biosynthesis

Sample Types

serum, plasma, tissue Homogenates

Detection Range

10 ng/mL-160 ng/mL

Sensitivity

10 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Target Details

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.

HGNC

20606

RGD

1309717

MGI

98715

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	92
	Range %	86-99
1:4	Average %	95
	Range %	87-105
1:8	Average %	90
	Range %	83-102

Recovery

The recovery of rat TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	88-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
10	2.553	2.568	2.561
20	1.748	1.763	1.756
40	0.885	0.875	0.880
80	0.515	0.511	0.513
160	0.285	0.287	0.286

References

Altered thyroid hormone production induced by long-term exposure to low doses of the endocrine disruptor dichlorodiphenyltrichloroethane Yaglova N V.et al,Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry,9(4): 339-342.
Mechanisms of Disruptive Action of Dichlorodiphenyltrichloroethane (DDT) on the Function of Thyroid Follicular Epitheliocytes. Yaglova NV. et al,Bull Exp Biol Med,2015 ,160(2):231-233
Rutin protects against aging-related metabolic dysfunction Li T. et al,Food Funct,2016 Jan 25.
ER stress contributes to high fat diet induced decrease of thyroglobulin and hypothyroidism Zhang X, et al,American Journal of Physiology-Endocrinology and Metabolism,January 8

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Function	Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Involvement in disease	Defects in Tg are a cause of a form of hypothyroidism in rdw rat.
Subcellular Location	Secreted
Protein Families	Type-B carboxylesterase/lipase family
Tissue Specificity	Thyroid gland specific.
Database Links	STRING: 10116.ENSRNOP00000009241 UniGene: Rn.10429
Pathway	Th17 cell differentiation Thyroid hormone synthesis

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