Product Details

Target Name

tryptophan hydroxylase 1

Alternative Names

Tph1 ELISA Kit; Tph ELISA Kit; Tryptophan 5-hydroxylase 1 ELISA Kit; EC 1.14.16.4 ELISA Kit; Tryptophan 5-monooxygenase 1 ELISA Kit

Abbreviation

TPH1

Uniprot No.

P09810

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

37.5 pg/mL-2400 pg/mL

Sensitivity

9.4 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	94-100
1:2	Average %	88
	Range %	83-96
1:4	Average %	92
	Range %	87-97
1:8	Average %	111
	Range %	106-115

Recovery

The recovery of rat TPH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-96
EDTA plasma (n=4)	105	103-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	1.821	1.831	1.826	1.692
1200	1.488	1.452	1.470	1.336
600	1.151	1.218	1.185	1.051
300	0.854	0.897	0.876	0.742
150	0.511	0.540	0.526	0.392
75	0.310	0.311	0.311	0.177
37.5	0.266	0.254	0.260	0.126
0	0.138	0.129	0.134

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

The Rat Tryptophan Hydroxylase (TPH) ELISA Kit is a powerful tool for quantitative analysis of the TPH protein in rat samples.

TPH is an important enzyme in the synthesis of serotonin, a neurotransmitter involved in various physiological and pathophysiological processes. This ELISA kit is specifically designed for the measurement of TPH protein levels in a variety of sample types, including serum, plasma, tissue homogenates, and cell lysates from Rattus norvegicus (Rat) species.

With a detection range of 37.5 pg/mL to 2400 pg/mL and a sensitivity of 9.4 pg/mL, this TPH ELISA kit can accurately detect even low levels of TPH protein. The assay principle is based on a sandwich immunoassay, which involves the use of two specific antibodies that bind to different epitopes of the target protein. This approach ensures high specificity and sensitivity in the measurement of TPH levels. The assay can be completed in just 1-5 hours, with a sample volume of 50-100ul, making it a fast and convenient option for research.

This ELISA kit provides a reliable and accurate method for the quantitative analysis of TPH protein levels in rat samples, making it an essential tool for any researcher studying the role of serotonin in various physiological and pathophysiological processes.

Target Background

Function
(From Uniprot)

Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.

Gene References into Functions

Ursolic acid derivatives targeted to tryptophan hydroxylase 1 prevented bone loss by suppressing serotonin biosynthesis without estrogenic side-effects in ovariectomized rats. PMID: 25930119
there exists possible interrelation between tyrosine hydroxylase and TPH gene expression and epigenetic histone acetylation in chronic unpredictable stress-induced depressive rats, which at least partly contributes to the etiology of depression. PMID: 24495952
An increased expression of TPH1 in the dorsal raphe nucleus may be the primary mechanism to explain the chronic increase in the brain synthesis of 5-HT in these in utero malnourished rats. PMID: 22819790
Transfection of VMAT-1 and TPH-1 induced vesicular storage of serotonin which is triggered release upon cooling and has protective effects against hypothermia. PMID: 22253933
An adjacent GC-rich cis -acting sequence cooperates with the inverted CCAAT box for full activation of basal transcription; both elements are essential for full cAMP response. In pinealocytes, NF-Y and Sp1 bind the inverted CCAAT box and GC-rich-region PMID: 12065627
regulation of rat tryptophan hydroxylase gene expression by stress involves 5' untranslated region PMID: 12153488
TPH1 mRNA expression was 105-fold more abundant in rat pineal than TPH2; TPH2 expression within the gland showed no significant variation with time of day and was very low. PMID: 12911638
This study reveals adrenal glucocorticoid-independent induction of tryptophan hydroxylase gene expression in raphe nuclei in response to immobilization stress. PMID: 14744469
TPH2 mRNA is expressed in raphe, in a pattern overlapping the histologically defined raphe nuclei. In sharp contrast, TPH1 (the previously known TPH) is expressed predominantly in pineal gland. PMID: 14960297
Tph1 and Tph2 transcripts showed robust diurnal rhythms of abundance, with highest levels at night. PMID: 15194882
Results show that the synthesis of 5-hydroxytryptamine and the expression of tryptophan hydroxylase in the dorsal raphe of rat offspring were suppressed by maternal ethanol intake. PMID: 16198203
TPH1 mRNA is 2.5-fold upregulated by the stress in dorsal raphe nucleus but not in pineal gland. PMID: 18197473
Data showed that calcium influx blockaded by nifedipine caused a decrease in tryptophan hydroxylase activity. PMID: 18221757
mRNA for TPH-1 was present in peripheral arteries, with low levels of TPH protein and TPH activity PMID: 18414394
data support the model that increased nocturnal 5-HT synthesis in the pineal gland is mediated by the phosphorylation of TPH1 at the serine 58, which elevates the TPH1 protein content and activity at night PMID: 18705647

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Protein Families

Biopterin-dependent aromatic amino acid hydroxylase family

Database Links

KEGG: rno:24848

STRING: 10116.ENSRNOP00000052959

UniGene: Rn.94788

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Code	CSB-E13984r
Size	96T,5×96T,10×96T
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Rat Tryptophan Hydroxylase (TPH) ELISA Kit

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Target Background