Rat Tumor necrosis factor β,TNF-β ELISA KIT

Instructions
Code CSB-E07379r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Tumor necrosis factor β,TNF-β
Alternative Names Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
Abbreviation TNF-β
Uniprot No. Q06332
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 101
Range % 96-105
1:2 Average % 102
Range % 97-107
1:4 Average % 87
Range % 83-94
1:8 Average % 96
Range % 91-102
Recovery
The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 99108
EDTA plasma (n=4) 88 84-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.632 2.765 2.699 2.614
250 2.212 2.302 2.257 2.172
125 1.545 1.577 1.561 1.476
62.5 0.765 0.771 0.768 0.683
31.2 0.411 0.423 0.417 0.332
15.6 0.257 0.264 0.261 0.176
7.8 0.176 0.185 0.181 0.096
0 0.084 0.085 0.085  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Gene References into Functions
  1. Adrenomedullin can increase the expression of TNF-beta and TNFR in renal early trauma. PMID: 20092780
  2. TNFbeta-so far only known to be produced by lymphocytes-was demonstrated in alveolar macrophages, bronchial epithelial cells, vascular smooth muscle cells and endothelial cells at the protein and the message level PMID: 12849708
Subcellular Location Secreted, Membrane
Protein Families Tumor necrosis factor family
Database Links

KEGG: rno:25008

STRING: 10116.ENSRNOP00000001111

UniGene: Rn.160577

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