Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Name: Rat Vasoactive Intestinal Peptide,VIP ELISA Kit
Brand: CUSABIO
SKU: CSB-E08355r
Price: 150 USD
Availability: InStock
Rating: 5.0 (3 reviews)

Product Details

Target Name

vasoactive intestinal peptide

Abbreviation

VIP

Uniprot No.

P01283

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.56 pg/mL-100 pg/mL

Sensitivity

0.39 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	97
	Range %	93-101
1:4	Average %	100
	Range %	97-103
1:8	Average %	86
	Range %	82-92

Recovery

The recovery of rat VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	86-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.384	2.506	2.445	2.324
50	1.732	1.813	1.773	1.652
25	1.112	1.100	1.106	0.985
12.5	0.615	0.626	0.621	0.500
6.25	0.386	0.397	0.392	0.271
3.12	0.297	0.286	0.292	0.171
1.56	0.196	0.193	0.195	0.074
0	0.121	0.120	0.121

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit is a highly sensitive and reliable assay for the quantification of VIP peptide in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat).

VIP peptide,a neuropeptide that is widely distributed in the central and peripheral nervous systems ,is known to play a critical role in the regulation of various physiological processes, particularly in the nervous system. Our ELISA kit is designed to accurately measure the levels of VIP peptides in biological samples, making it an essential tool for researchers in the field of neuroscience.

The detection range of the kit is 1.56 pg/mL-100 pg/mL, with a sensitivity of 0.39 pg/mL, ensuring precise and accurate quantification of VIP peptides in samples. The assay time is only 1-5 hours, and the sample volume required is minimal (50-100ul), making the kit highly efficient and suitable for high-throughput experiments.

The VIP ELISA kit uses a sandwich assay principle, which involves the use of a VIP-specific antibody to capture the peptide of interest, followed by the detection of the captured VIP peptide using a second VIP-specific antibody. The detection wavelength is 450 nm, and the results are easily obtained using a microplate reader.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 3 reviews

Submit a Review here

Sample type: Serum

Sample species: Rat

Sample dilution: : 1:2

Review: I diluted the rat serum at a 1:2 dilution ratio, and the results obtained were as expected, very much as expected. The kit reagents are stable, the measured results are reasonable, and there was no fraud.

By Anonymous

Sample type: Serum

Sample species: Rat

Sample dilution: : 1:5

Review: I used this kit (catalog number CSB-E08355r) to detect rat serum samples. The samples were operated after a 1:5 dilution. The OD value linear relationship of the standard curve was good, with a correlation coefficient of 0.996, and the detection results had excellent repeatability. The kit has clear operating steps and stable reagent activity, with no performance degradation when stored at 2-8°C. It has strong detection specificity for rat VIP, no obvious cross-reactivity, and fully meets the quantitative needs of scientific research experiments. It is a cost-effective ELISA kit.

By Anonymous

Sample type: Serum Tissue homogenate Intestine

Sample species: Rat

Sample dilution: : 1:5

Review: I used CSB-E08355r to detect the concentration of vasoactive intestinal peptide (VIP) in rat tissue homogenate and rat serum. The OD value of the standard curve was around 1.0, and the OD values of the samples were between 0.1 and 1.0, with all results within the linear range.

By Anonymous

Code	CSB-E08355r
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.