Recombinant Rat VIP peptides (Vip)

Code CSB-YP355621RA
MSDS
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Source Yeast
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Code CSB-EP355621RA
MSDS
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Source E.coli
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Code CSB-EP355621RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP355621RA
MSDS
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Source Baculovirus
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Code CSB-MP355621RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Vip
Uniprot No.
Alternative Names
VipVIP peptides [Cleaved into: Intestinal peptide PHV-42; Intestinal peptide PHI-27; Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide; VIP; Vasoactive intestinal polypeptide)]
Species
Rattus norvegicus (Rat)
Expression Region
81-122
Target Protein Sequence
HADGVFTSDY SRLLGQISAK KYLESLIGKR ISSSISEDPV PV
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.; PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin. PHI also causes vasodilation.
Gene References into Functions
  1. findings indicate that PACAP and VIP can prevent the deleterious effect of Oxygen/Glucose Deprivation on rat neuronal cells. PMID: 25712659
  2. VIP is an important regulator of cardiovascular and digestive system in physiological and pathological conditions PMID: 25990439
  3. Data support the role of VIP as a local neuromodulator released by intrinsic enteric neurons and responsible for the induction of IGN through a VPAC1 receptor-dependent mechanism in enterocytes. PMID: 25586379
  4. Alterations in VIP expression may play a role in trinitrobenzene sulfonic acid-induced colitis. PMID: 25574088
  5. Data suggest long-term dietary L-arginine supplementation up-regulates VIP and eNOS (endothelial nitric oxide synthase) in mucosa small intestine (particularly in enterocytes and interstitial cells of Cajal) undergoing adaptation to cold temperature. PMID: 24100601
  6. Stimulating hippocampal microglia with the peptide neurotransmitter VIP via VPAC1 receptors increases production of IL-4 which promotes their trophic and proliferative effects on neural stem/progenitor cells PMID: 24801739
  7. Our findings point to a predominant involvement of the right hippocampal CA1 area in the positive effect of VIP on learning and memory impairments of rats with an OBX model of depression rats PMID: 22160165
  8. VIP had protective effects on severe acute pancreatitis gut barrier function. PMID: 21725849
  9. VIP was found in many neurons and fibers of the sphenopalatine ganglion PMID: 22108610
  10. Treatment with VIP reduces brain damage in ischemic rats, an effect that may be associated with attenuation of apoptosis and expression of S100 protein beta (S100B) polypeptide. PMID: 21620378
  11. Report age-related changes in myosin-V myenteric neurons, CGRP and VIP immunoreactivity in the ileum of rats supplemented with ascorbic acid. PMID: 22127604
  12. PACAP and VIP induce morphological and functional differentiation of embryonic stem cells into a neuronal phenotype PMID: 22001490
  13. Estradiol attenuated acute lung injury in three experimental models while stimulating VIP gene expression PMID: 22009726
  14. These results suggest that the nitric oxide-cyclic GMP pathway differentially regulates vasoactive intestinal peptide in the dentate gyrus DG and hippocampus during seizure. PMID: 20369387
  15. VIP elevated SP-A expression in ATII cells which was mediated by enhanced sp-a gene transcription PMID: 20688121
  16. Conclude that the spontaneous release of VIP from the enteric motor neurons maintains homeostasis in smooth muscle function in mild inflammation by blocking the activation of NF-kappaB. PMID: 19661154
  17. Data show that the light synchronization process in the suprachiasmatic nucleus involves a selective remodelling of synapses at sites of photic integration, including VIP neurons. PMID: 20074215
  18. voltage-gated Ca(2+) channels (VGCCs) responsible for the non-adrenergic non-cholinergic (NANC) relaxation and vasoactive intestinal polypeptide (VIP)-like immunoreactivity release in the rat gastric fundus PMID: 19945453
  19. Cultured female rat pituitary cells were treated with 10 nM 1,17 beta-estradiol or triiodothyronine. E2 treatment for 3 days resulted in a 5-fold increase in VIP mRNA. In contrast, T(3) treatment reduced the mRNA levels of VIP to 40% of control values. PMID: 12147214
  20. In the basolateral amygdala, VIP-positive (VIP+) axon terminals form synaptic-like contacts with calbindin-positive (CB+) neurons, and with pyramidal neurons. PMID: 12528187
  21. VIP promoted survival of myenteric neurons in culture. PMID: 12609771
  22. Changes of substance P and VIP levels in initial part of ileum and blood of spleen deficiency rats and cool stress rats may be closely related to the gastrointestinal motility disorders presented in spleen deficiency and cool stress rats. PMID: 12632517
  23. Not only does VIP alter the intrathalamic rhythmic activity, this peptide that is endogenous to the thalamic circuit may also play a significant role in the regulation of information transfer through the thalamocortical circuit. PMID: 12711712
  24. Increases in the spinal levels of VIP and NPY after peripheral nerve injury were not sufficient for the development of neuropathic pain. PMID: 12727330
  25. Pituitary VIP mediates the effect of estrogen on lactotroph hyperplasia, pituitary TGF-beta1, and angiogenesis. PMID: 12960047
  26. Plasma VIP significantly decreased at postinjury 3, 12 and 24 hours, but significantly increased at 72 hours and declined on day 7. In jejunum significantly increased at 3 hours postinjury and declined at 12 and 24 hours, but increased at 72 hours. PMID: 15040036
  27. The increased nonadrenergic noncholinergic and the decreased effects of VIP in mediating inhibitory nerves may largely relate to the peristaltic abnormalities seen in the isografted LEW rat jejunum. PMID: 15050161
  28. Aging reduces vasoactive intestinal peptide (VIP) receptor mRNA and eliminates diurnal expression of VIP mRNA within the suprachiasmatic nucleus of aged male rats. PMID: 15344914
  29. VIP can modulate not only immune responses but also hormonal responses during acute inflammation. PMID: 15467350
  30. VIP had a modulatory action on jejunal potassium absorption when injected into the nucleus tractus solitarius. PMID: 15627496
  31. Significant changes in NPY and VIP prostate nerve fibers, showing an increase in periurethral ducts at sex maturation. PMID: 15716245
  32. Involved in regulating the proliferation of prolactin-secreting cells, in an in vitro study on monolayer cultures of rat pituitary cells. PMID: 16328361
  33. Intra-articular injection of VIP into normal rat knee joints caused pain responses & VPAC antagonists abolished them. VIP may be involved in the generation of osteoarthritis pain. PMID: 16564620
  34. pituitary adenylate cyclase-activating polypeptidergic mechanisms within the pontine reticular formation play a critical role in long-term regulation of rapid eye movement sleep PMID: 16888155
  35. adenylate cyclase activating polypeptide and vasoactive intestinal polypeptide are differentially regulated and are involved in the regulatory processes under a food-restricted regimen, and are differentially altered in nocturnal and diurnal species PMID: 16888191
  36. Activation of c-Jun in dorsal root ganglia induces VIP and NPY upregulation and contributes to pathogenesis of neuropathic pain following chronic constriction injury. PMID: 17081520
  37. Small bowel transplantation (SBT) decreased response to exogenous Substance P, although release of endogenous Substance P (EFS) is preserved. Changes in VIP signaling are acute and reversible and not caused by effects of SBT PMID: 17512547
  38. Footshock stress decreased the number of cells expressing VIP mRNA in the SCN without changing hybridization level per cell. PMID: 17826907
  39. Chronic atropine administration diminishes the contribution of vasoactive intestinal polypeptide to heart rate regulation. PMID: 18052688
  40. Hippocampal asymmetry in exploratory behavior to Vip is reported. PMID: 18321609
  41. Data demonstrate PACAP and PAC1, but not VIP, regulation in micturition pathways with inflammation and PACAP-mediated ATP release from urothelium. PMID: 18563302
  42. Data show that the role of VIP in claustral development is assoicated with the regulation of neuronal proliferation and differentiation. PMID: 18598846
  43. Data show that in enteric nerves anandamide stimulates VIP release by activation of a CB2 receptor specific pathway, while the stimulation of NO production suggests the existence of an additional type of cannabinoid receptor in the enteric nervous system. PMID: 18829105
  44. Our results suggested that both PACAP38 and VIP could act as trophic factors in Schwann cells. PMID: 18835258
  45. Release of regulatory gut peptide vasoactive intestinal peptide by acid and hyperosmolal solutions in the intestine in conscious rats PMID: 18992283
  46. The results showed that VIP, PACAP-38, PACAP-27 and the VPAC(1) specific agonist evoked significant dilations with the rank order of potency; VIP = PACAP-38 > PACAP-27 = [Lys15, Arg16, Leu27]-VIP(1-7)-GRF(8-27). PMID: 19220306
  47. Treatment with VIP enhances angiogenesis in the ischemic brain, and this effect may be mediated by increases in levels of VEGF and its receptors. PMID: 19332106

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Subcellular Location
Secreted.
Protein Families
Glucagon family
Database Links
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