Rat advanced oxidation protein products(AOPP) ELISA kit

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Rat advanced oxidation protein products(AOPP) ELISA kit

Citations (7) Protocol MSDS

Code	CSB-EQ027429RA
Size	96T,5×96T,10×96T
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Product Details
Citations (7)
Customer Reviews and Q&A

Target Name

advanced oxidation protein products(AOPP)

Abbreviation

AOPP

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 nmol/mL-1000 nmol/mL

Sensitivity

3.9 nmol/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat AOPP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-92
1:2	Average %	91
	Range %	86-96
1:4	Average %	87
	Range %	83-93
1:8	Average %	98
	Range %	91-104

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml

OD1

OD2

Average

Corrected

1000

3.040

2.934

2.987

2.835

500

2.492

2.367

2.430

2.278

250

1.746

1.828

1.787

1.636

125

1.027

1.019

1.023

0.871

62.5

0.679

0.691

0.685

0.533

31.2

0.436

0.453

0.444

0.293

15.6

0.324

0.304

0.314

0.163

0.148

0.155

0.152

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat AOPP ELISA Kit was designed for the quantitative measurement of Rat AOPP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 nmol/mL-1000 nmol/mL and the sensitivity is 3.9 nmol/mL.

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Combined effect of pantoprazole and mesenchymal stem cells on experimentally induced gastric ulcer: implication of oxidative stress, inflammation and apoptosis pathways AH Sayed,Inflammopharmacology,2024
Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods P Xie,Poultry Science,2022
Possible curative role of curcumin and silymarin against nephrotoxicity induced by gamma-rays in rats Nadia Abdel-Magied, et al,Experimental and Molecular Pathology,2019
Zhen-wu-tang ameliorates adenine-induced chronic renal failure in rats: regulation of the canonical Wnt4/beta-catenin signaling in the kidneys La L.et al,J Ethnopharmacol,2017
Antioxidant therapy improves non-thyroidal illness syndrome in uremic rats Yang P.et al,Ren Fail.,2016
Dexmedetomidine acts as an oxidative damage prophylactic in rats exposed to ionizing radiation Dilek Kutanis.et al,Journal of Clinical Anesthesia,2016

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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