Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

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Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Name: Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Brand: CUSABIO
SKU: CSB-E10786r

^Instructions

Code	CSB-E10786r
Size	96T,5×96T,10×96T
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Target Name

amyloid beta peptide 1-42,Aβ1-42

Abbreviation

Aβ1-42

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.9 pg/mL-60 pg/mL

Sensitivity

0.225 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-90
1:2	Average %	94
	Range %	90-98
1:4	Average %	101
	Range %	97-104
1:8	Average %	96
	Range %	93-99

Recovery

The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	100	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
60	2.281	2.274	2.278	2.136
30	1.599	1.576	1.588	1.446
15	0.967	0.924	0.946	0.804
7.5	0.538	0.528	0.533	0.391
3.75	0.395	0.371	0.383	0.241
1.8	0.321	0.312	0.317	0.175
0.9	0.235	0.250	0.243	0.101
0	0.141	0.143	0.142

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Combination of Omega 3 and Coenzyme Q10 Exerts Neuroprotective Potential Against Hypercholesterolemia-Induced Alzheimer's-Like Disease in Rats GI Fouad,Neurochemical Research,2020
Gagandeep Kaur Atish Prakash, et al,The Journal of Nutritional Biochemistry,2019
Effect of Berberine and Ipriflavone Mixture against Scopolamine-Induced Alzheimer-Like Disease Mohamed S. Abdel-Latif, et al,International Journal of Pharmaceutical and Phytopharmacological Research,2019
The potential value of capsaicin in modulating cognitive functions in a rat model of streptozotocin-induced Alzheimer’s disease Manal A. Shalaby, et al,Febs Journal,2019
Neuroprotective Effects of Zafirlukast, Piracetam and Their Combination on L-Methionine-Induced Vascular Dementia in Rats Fayez AM, et al,fundamental & clinical pharmacology,2019
Treadmill Exercise Ameliorates Spatial Learning and Memory Deficits Through Improving the Clearance of Peripheral and Central Amyloid-Beta Levels Davar Khodadadi.et al,Neurochemical Research,2018
Catalpol provides a protective effect on fibrillary Aβ1-42 -induced barrier disruption in an in vitro model of the blood–brain barrier Liu C.et al,Phytother Res,2018
Possible role of endothelin receptor against hyperhomocysteinemia and B-amyloid induced AD type of vascular dementia in rats Singh M.et al,Brain Res Bull.,2017
Neuroprotective effect of berberine against environmental heavy metals-induced neurotixicity and Alzheimer's-like disease in rats Hend M.Hussien.et al,Food and Chemical Toxicology,2017
Developmental perfluorooctane sulfonate exposure results in tau hyperphosphorylation and B-amyloid aggregation in adults rats: Incidence for link to Alzheimer's disease Zhang Q.et al,Toxicology.,2016
Modulation of LOX and COX pathways via inhibition of amyloidogenesis contributes to mitoprotection against B-amyloid oligomer-induced toxicity in an animal model of Alzheimer's disease in rats. Kalra J.et al,Pharmacol Biochem Behav.,2016
Berberine Reduces Neurotoxicity Related to Nonalcoholic Steatohepatitis in Rats Ghareeb DA. et al,Evid Based Complement Alternat Med,2015

■ Q&A

Please provide the following information regarding Rat amyloid beta peptide 1-42, Abeta1-42 ELISA Kit (CSB-E10786r)
a. Is this soluble or insoluble Abeta1-42?
b. Also, can this kit be used with plasma and brain tissue from rat?

Thanks for your inquiry.
This kit can detect soluble Abeta1-42，and it can be used with plasma and brain tissue from rat.
Pls let me know if you have any further questions. Thank you.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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