Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

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Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Specification

Code

CSB-E10786r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Lead Time

3-5 working days

Abbreviation

Aβ1-42

Target Name

amyloid beta peptide 1-42,Aβ1-42

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.9 pg/mL-60 pg/mL

Sensitivity

0.225 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-90
1:2	Average %	94
	Range %	90-98
1:4	Average %	101
	Range %	97-104
1:8	Average %	96
	Range %	93-99

Recovery

The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	100	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
60	2.281	2.274	2.278	2.136
30	1.599	1.576	1.588	1.446
15	0.967	0.924	0.946	0.804
7.5	0.538	0.528	0.533	0.391
3.75	0.395	0.371	0.383	0.241
1.8	0.321	0.312	0.317	0.175
0.9	0.235	0.250	0.243	0.101
0	0.141	0.143	0.142

References

Berberine Reduces Neurotoxicity Related to Nonalcoholic Steatohepatitis in Rats Ghareeb DA. et al,Evid Based Complement Alternat Med,2015 (2015 ),13 pages
Modulation of LOX and COX pathways via inhibition of amyloidogenesis contributes to mitoprotection against B-amyloid oligomer-induced toxicity in an animal model of Alzheimer's disease in rats. Kalra J.et al,Pharmacol Biochem Behav.,146-147:1-12
Developmental perfluorooctane sulfonate exposure results in tau hyperphosphorylation and B-amyloid aggregation in adults rats: Incidence for link to Alzheimer's disease Zhang Q.et al,Toxicology.,2016 347-349:40-6.
Neuroprotective effect of berberine against environmental heavy metals-induced neurotixicity and Alzheimer's-like disease in rats Hend M.Hussien.et al,Food and Chemical Toxicology,Nov 21
Possible role of endothelin receptor against hyperhomocysteinemia and B-amyloid induced AD type of vascular dementia in rats Singh M.et al,Brain Res Bull.,Mar 6
Catalpol provides a protective effect on fibrillary Aβ1-42 -induced barrier disruption in an in vitro model of the blood–brain barrier Liu C.et al,Phytother Res,Feb 26
Treadmill Exercise Ameliorates Spatial Learning and Memory Deficits Through Improving the Clearance of Peripheral and Central Amyloid-Beta Levels Davar Khodadadi.et al,Neurochemical Research,June 11
Neuroprotective Effects of Zafirlukast, Piracetam and Their Combination on L-Methionine-Induced Vascular Dementia in Rats Fayez AM, et al,fundamental & clinical pharmacology,April 18

Still Have Questions?

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Q&A

Please provide the following information regarding Rat amyloid beta peptide 1-42, Abeta1-42 ELISA Kit (CSB-E10786r)
a. Is this soluble or insoluble Abeta1-42?
b. Also, can this kit be used with plasma and brain tissue from rat?

Thanks for your inquiry.
This kit can detect soluble Abeta1-42，and it can be used with plasma and brain tissue from rat.
Pls let me know if you have any further questions. Thank you.

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Aβ1-42 ELISA Kit

Aβ1-42 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E10684h	Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit	serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)	0.078 ng/mL

Aβ1-42 ELISA Kit for Monkey

Code	Product Name	Sample Types	Sensitivity
CSB-E14398Mk	Monkey amyloid beta peptide 1-42,Aβ1-42 ELISA Kit	serum, plasma	0.039 ng/mL

Aβ1-42 ELISA Kit for Mus musculus (Mouse)

Code	Product Name	Sample Types	Sensitivity
CSB-E10787m	Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit	serum, plasma, tissue homogenates	3.9 pg/mL

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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