Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Code CSB-E10786r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat Aβ1-42 ELISA Kit was designed for the quantitative measurement of Rat Aβ1-42 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 pg/mL-60 pg/mL and the sensitivity is 0.225 pg/mL .

Target Name amyloid beta peptide 1-42,Aβ1-42
Abbreviation Aβ1-42
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.9 pg/mL-60 pg/mL
Sensitivity 0.225 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %83-90
1:2Average %94
Range %90-98
1:4Average %101
Range %97-104
1:8Average %96
Range %93-99
Recovery
The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
602.281 2.274 2.278 2.136
301.599 1.576 1.588 1.446
150.967 0.924 0.946 0.804
7.50.538 0.528 0.533 0.391
3.750.395 0.371 0.383 0.241
1.80.321 0.312 0.317 0.175
0.90.235 0.250 0.243 0.101
00.141 0.143 0.142  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Please provide the following information regarding Rat amyloid beta peptide 1-42, Abeta1-42 ELISA Kit (CSB-E10786r)
a. Is this soluble or insoluble Abeta1-42?
b. Also, can this kit be used with plasma and brain tissue from rat?

A:
Thanks for your inquiry.
This kit can detect soluble Abeta1-42,and it can be used with plasma and brain tissue from rat.
Pls let me know if you have any further questions. Thank you.
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