Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Code CSB-E10786r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Aβ1-42
Target Name amyloid beta peptide 1-42,Aβ1-42
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.9 pg/mL-60 pg/mL
Sensitivity 0.225 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %83-90
1:2Average %94
Range %90-98
1:4Average %101
Range %97-104
1:8Average %96
Range %93-99
Recovery
The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
602.281 2.274 2.278 2.136
301.599 1.576 1.588 1.446
150.967 0.924 0.946 0.804
7.50.538 0.528 0.533 0.391
3.750.395 0.371 0.383 0.241
1.80.321 0.312 0.317 0.175
0.90.235 0.250 0.243 0.101
00.141 0.143 0.142  
References
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Q&A and Customer Reviews

Q&A
Q:

Please provide the following information regarding Rat amyloid beta peptide 1-42, Abeta1-42 ELISA Kit (CSB-E10786r)
a. Is this soluble or insoluble Abeta1-42?
b. Also, can this kit be used with plasma and brain tissue from rat?

A:
Thanks for your inquiry.
This kit can detect soluble Abeta1-42,and it can be used with plasma and brain tissue from rat.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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