Rat angiotensinogen (aGT) ELISA Kit

Instructions
Code CSB-E08565r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Alternative Names Agt ELISA Kit; Serpina8Angiotensinogen ELISA Kit; Serpin A8) [Cleaved into: Angiotensin-1 ELISA Kit; Angiotensin 1-10 ELISA Kit; Angiotensin I ELISA Kit; Ang I); Angiotensin-2 ELISA Kit; Angiotensin 1-8 ELISA Kit; Angiotensin II ELISA Kit; Ang II); Angiotensin-3 ELISA Kit; Angiotensin 2-8 ELISA Kit; Angiotensin III ELISA Kit; Ang III ELISA Kit; Des-Asp[1]-angiotensin II); Angiotensin-4 ELISA Kit; Angiotensin 3-8 ELISA Kit; Angiotensin IV ELISA Kit; Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4] ELISA Kit
Abbreviation AGT
Uniprot No. P01015
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 107    
Range % 100-111    
1:200 Average % 104    
Range % 97-108    
1:400 Average % 90    
Range % 83-94    
1:800 Average % 94    
Range % 85-97    
Recovery
The recovery of rat aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 91 85-95    
EDTA plasma (n=4) 101 93-104    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2000 2.204 2.099 2.152 2.015    
1000 1.744 1.713 1.729 1.592    
500 1.098 1.067 1.083 0.946    
250 0.605 0.617 0.611 0.474    
125 0.431 0.421 0.426 0.289    
62.5 0.277 0.266 0.272 0.135    
31.25 0.175 0.181 0.178 0.041    
0 0.138 0.136 0.137      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
Gene References into Functions
  1. these results suggest that 2K1C rats are more responsive to the natriorexigenic effects of ANG II, whereas other responses to ANG II are not modified. PMID: 29305157
  2. CGRP has an anti-inflammatory effect in vascular smooth muscle cells (VSMCs) stimulated by Ang II; ADAM17 is involved in the protective effect of CGRP against Ang II-induced inflammation via the EGFR-ERK1/2 pathway in VSMCs PMID: 30046277
  3. Ang II induced epithelial-mesenchymal transition in renal tubular epithelial cells via Tgfb1. PMID: 29746862
  4. The authors provide evidence demonstrating the critical role of Angiotensin II/TRPC6 axis in the control of glomeruli function, which is likely important for the development of glomerular diseases. PMID: 28331185
  5. Ang II down-regulates KLF15 expression via the AT1 receptor, and KLF15 is likely to inhibit Ang II-induced CTGF expression by repressing the recruitment of the co-activator P/CAF to the CTGF promoter. PMID: 29179208
  6. results established that A20 is involved in the renoprotective effect by calcitriol via negatively modulating the NF-kappaB pathway and necroptotic pathway in AngII-induced renal injury. PMID: 28744711
  7. The mRNA expression of cardiac AngII was significantly increased after the 8week exercise period, while AT1R was significantly decreased. PMID: 28656283
  8. ACE2Ang (17)MasR axis may present a potential protective role in the development of myocardial remodeling. PMID: 28656296
  9. CaSR activation results in intracellular calcium release, MEK1/2 pathway phosphorylation, autophagy activation and collagen formation induced by AngII in cardiac fibroblasts. PMID: 29452090
  10. Ang II is involved in the degradation of myosin light chain kinase in pressure overload-induced cardiac hypertrophy and that this process was mediated by MMP9. PMID: 29262413
  11. Data suggest that increases in adrenal blood flow induced by Ang II (angiotensin II)/Agtr2 signaling are mediated by release of nitric oxide and EETs (epoxyeicosatrienoic acids); increases in adrenal blood flow with ACTH (adrenocorticotropic hormone)/Mc2r receptor signaling are mediated by EETs only. (Agtr2 = angiotensin II receptor; Mc2r = ACTH receptor) PMID: 29140411
  12. Ang II increased Csk expression and induced podocyte apoptosis, stimulated Csk translocation and binding to Caveolin-1. PMID: 27225249
  13. Major components of the renin angiotensin system are located in striatal projection neurons, and striatal astrocytes and microglial cells of rats. PMID: 28161727
  14. Ang-(1-5) is an active mediator of renin-angiotensin system to stimulate ANP secretion via Mas R and PI3K-Akt-NOS pathway. PMID: 27660028
  15. Smad7 influenced NF-kappaB expression by regulating reactive oxygen species generation, and induced glomerular mesangial cell apoptosis to counter the Ang II-promoted proliferation. PMID: 28988741
  16. The activation of Ang II system in nucleus tractus solitarii may impair blood pressure baroreflex, and contribute to autonomic dysreflexia after spinal cord injury. PMID: 28742157
  17. Enhanced expression and phosphorylation of Sirt7 activates smad2 and ERK signaling and promotes the cardiac fibrosis differentiation upon angiotensin-II stimulation. PMID: 28582407
  18. Ang II upregulates the expression of Kv1.5, SAP97 and TGF-beta1 in neonatal rat atrial myocytes. PMID: 25451261
  19. MiRNA-29a and AGT mRNA expressions were negatively correlated in a rat model of diabetic retinopathy (DR). Overexpression of miRNA-29a could down-regulate AGT expression, thereby preventing the development of DR. PMID: 28189547
  20. Results indicate that aldosterone (Aldo)-mineralocorticoid receptor (MR) signaling in the subfornical organ (SFO) is needed for the activation of angiotensin II-AT1R-reactive oxygen species signaling from the SFO to the nucleus (PVN)and rostral ventrolateral medulla. Activation of Aldo-MR signaling from the SFO to the supraoptic nucleus may enhance AT1R dependent activation of pre-sympathetic neurons in the PVN. PMID: 27163380
  21. Ang-(1-7) improved liver fibrosis by regulating NLRP3 inflammasome activation induced by Ang II-mediated reactive oxygen species via redox balance modulation. PMID: 26728324
  22. ANG II-induced IL-6 elevation in macrophages enhances activation of the JAK-STAT pathway and consequent angiotensinogen upregulation in proximal tubule cells, suggesting involvement of an immune response in driving intrarenal renin-angiotensin system activity PMID: 27009340
  23. intracellular ANG II stimulates AGT expression in the proximal tubules, leading to increased AGT formation and secretion into the tubular fluid, which contributes to ANG II-dependent hypertension PMID: 26864937
  24. Findings highlight the complexity of angiotensinogen (AGT)regulation and show that AGT protein and mRNA levels are responsive to angiotensin II in both SHR and Wistar astrocytes. Most importantly, our findings suggest that this peptide can induce its own synthesis by positively regulating AGT protein synthesis. PMID: 27131988
  25. CTRP6 improves PPARgamma activation and alleviates AngII-induced hypertension and vascular endothelial dysfunction PMID: 27871858
  26. H2S downregulates Ang II-induced atrial Kv1.5 expression by attenuating Nox4-related ROS-triggered P-Smad2/3 and P-ERK 1/2 activation during atrial fibrillation PMID: 28011270
  27. Compared to chymase, ACE-mediated angiotensin-(1-12) metabolism in the heart was several fold lower. Overall our findings suggest that angiotensin-(1-12), not Ang I, is the better substrate for Ang II formation by chymase in adult rats. PMID: 27465904
  28. glomerular expression of Renin-angiotensin system components in nephrotic models, was examined. PMID: 27932705
  29. These observations demonstrate that the effect of Ang-(1-12) on potassium current was related to the formation of Ang II and that the peptide has arrhythmogenic properties. PMID: 27590241
  30. enhancing Ang-(1-7)-Mas-R-nNOS system is likely to be beneficial in preventing against cardiovascular and cerebrovascular dysfunction. PMID: 27960152
  31. overproduction of O2 (-) through overexpression of CuZnSOD in the organum vasculosum of the lamina terminalis plays an important role in the development of chronic AngII-dependent hypertension PMID: 26881025
  32. The results of this study suggested that disruption of astroglial AOGEN synthesis leads to cardiovascular, cognitive, behavioral, and sleep disorders that might be partly due to LC dysfunction. PMID: 26775713
  33. A reciprocal regulatory relationship between DDR2 and collagen, involving cross-talk between the GPCR and RTK pathways, is central to Angiotensin II-induced increase in collagen expression in cardiac fibroblasts. PMID: 26674152
  34. Urinary AGT elevation prior to urinary protein levels suggests that AGT is an early biomarker of kidney dysfunction in intrauterine growth restricted offspring rats. PMID: 26270574
  35. The antiaging protein klotho inhibits angiotensin II -induced cardiomyocyte hypertrophy. PMID: 26970306
  36. PAR1 and PAR2 play a critical role in Ang II-induced adventitial fibroblasts activation, and this may contribute to adventitia-related pathological changes. PMID: 27012211
  37. Angiotensin II modifies carotid body chemosensitivity, suggest that the increased circulating levels of ANG II and leptin induced by intermittent hypoxia act at the carotid body to alter leptin signaling within the carotid body which in turn may influence chemoreceptor function. PMID: 26431621
  38. Data suggest that valsartan may decrease the secretion of transforming growth factor (TGF-beta) and fibronectin (FN) in a dose-dependent manner via inhibiting angiotensin II-notch1 receptor (AngII-Notch) signaling. PMID: 26728369
  39. Ang II promotes activation of ARF6 to controls reactive oxygen species production by regulating Rac1 activation and Nox1 expression. PMID: 26824355
  40. PGC-1-alpha inhibits AngII-induced vascular smooth muscle cells proliferation and migration. PMID: 26310573
  41. the augmented AGT released from apoptotic endothelial cells acts as a vital progenitor of Ang II to accelerate vascular remodelling PMID: 26147666
  42. miR-7a/b has an anti-fibrotic role in ANG II-treated CFs that is mediated by Sp1 mechanism involving the TGF-beta and MAPKs pathways. PMID: 25923922
  43. Compared to LIT, HIIT resulted in reduced Agt mRNA expression (p = 0.035), reduced plasma LDL (p = 0.001), triglycerides (p = 0.029), and total cholesterol (p = 0.002), increased plasma albumin (p = 0.047), reduced remnant kidney weight (p = 0.005) PMID: 26090382
  44. the effect of leptin treatment for 7 and 28 days on renal function and morphology and the participation of angiotensin II (Ang II), through its AT1 receptor, was examined. PMID: 25793389
  45. Ang II plays a role in Venezuelan equine encephalitis and that brain inflammation is protective against viral infection. PMID: 26156105
  46. Overexpression of clusterin in proximal tubular epithelial cells decreased the levels of Ang II-stimulated fibrotic markers and AT1R. PMID: 25148511
  47. These results suggest that Ang-(1-7) increases DUSP1 to reduce MAP kinase/Smad/CTGF signaling and decrease fibrosis in resistance arterioles, to attenuate end-organ damage associated with chronic hypertension. PMID: 25079175
  48. In diabetic rats, renal angiotensinogen is positively associated with diabetic albuminuria and contributes to the sex-dependent differences in renal injury. PMID: 25529203
  49. findings suggest that an increased concentrations of Ang peptides in the systemic circulation during exercise may promote neurogenesis in the adult rat hippocampus PMID: 25223907
  50. Ang-(1-7) abolished advanced glycated end product-induced activation of the MAP kinase ERK1/2. PMID: 25246357
  51. Ang II synergized with high glucose in the release of pro-inflammatory factors mainly through the upregulation of TLR4 signaling in mesangial cells in the pathogenesis of diabetic nephropathy PMID: 25732086
  52. data suggest that pharmacological doses of relaxin do not reverse severe effects of Ang II. PMID: 24710077
  53. Cell atavistic transition: Paired box 2 re-expression occurs in mature tubular epithelial cells during acute kidney injury and is regulated by Angiotensin II. PMID: 24710423
  54. Angiotensin-(1-7) induces cerebral ischaemic tolerance by promoting brain angiogenesis in a Mas/eNOS-dependent pathway PMID: 24824997
  55. All-trans retinoic acid acts as a positive regulator of PHB1, PHB2 and ACE2, and as a negative regulator of ACE1, angiotensin I, and angiotensin II in a renal tubular epithelial cells model system under hypoxia/reoxygenation conditions. PMID: 25031298
  56. present study suggests that gAcrp could ameliorate AngII-induced cardiac hypertrophy and fibrosis in rat atrial cells PMID: 25099270
  57. AngII induced STAT3 activation and pro-fibrotic responses in cultured renal tubular epithelial cells. PMID: 24976155
  58. SLC41A1 knockdown inhibits angiotensin II-induced cardiac fibrosis by preventing Mg(2+) efflux and Ca(2+) signaling in cardiac fibroblasts. PMID: 25263961
  59. Mas-mediated antioxidant effects also restored diabetic rat carotid flow, pointing to the contribution of ACE2-angiotensin-(1-7)-Mas axis in maintaining carotid flow. PMID: 24877125
  60. Angiotensin receptor blockade and inhibition of local AngII production through angiotensin-converting enzyme inhibition prevented glucose-mediated increased TRPC6 expression. PMID: 24731445
  61. The augmented angiotensinogen mRNA levels in segment 3 and angiotensinogen protein levels in segments 1 and 3 of renal proximal tubules may contribute to the progression of diabetic nephropathy. PMID: 24910532
  62. Complexes of angiotensin II with functionally different proteins are differentially and simultaneously involved in compensation of behavioral and hemodynamic disturbances during acute and/or chronic hypoglycemia and hyperglycemia. PMID: 24771417
  63. Methylglyoxal activates NF-kappaB through RAGE and thereby increases renin/angiotensin/aldosterone levels and blood pressure. PMID: 24436324
  64. The immunoexpression of Ang II and AT1/AT2 was altered in activated microglia notably at 1 week in rats subjected to middle cerebral artery occlusion (MCAO). PMID: 23754618
  65. Endogenous (systemic and/or local) Ang 1-7 regulates insulin-mediated glucose transport in vivo. PMID: 24184594
  66. Data indicate that neither oophorectomy (OVX) nor OVX+17 beta-estradiol (E2) altered cardiac expression or activity of renin, angiotensinogen. PMID: 24204720
  67. Angiotensin II-induced activation of central AT1 receptors exerts endocannabinoid-mediated gastroprotective effect in rats. PMID: 24145131
  68. Neurolysin was identified as a non-AT1 and non-AT2 binding site for Angiotensin II. PMID: 23412923
  69. Lipid rafts couple to angiotensinogen gene expression through a mechanism that involves beta1-integrin. PMID: 23058350
  70. The insulin-angiotensin II interaction at the hypothalamic level might be involved in the increase in blood pressure observed in the insulin resistant state. PMID: 23816464
  71. beta carotene may have protective effective on chronic renal disorder. It may repress the inflammatory genes (Ren 1, PPARgamma) to achieve the protective effect on Ang II induced renal damage. PMID: 23117547
  72. Pro)renin receptor and insulin resistance: possible roles of angiotensin II-dependent and -independent pathways. [review] PMID: 22684035
  73. Hypoxia in cardiomyocytes increases UII and collagen I expression through the induction of AngII, ROS, and the JNK pathway causing cardiomyocyte hypertrophy and fibrosis. PMID: 24481965
  74. We demonstrate that stimulatory signals can change the DNA methylation status at a CCAAT/enhancer binding protein (CEBP) binding site and a transcription start site and activate expression of the angiotensinogen gene (AGT). PMID: 24191285
  75. Disruption of the blood-brain barrier results in increased activation of tyrosine hydroxylase-containing cells by circulating angiotensin II. PMID: 23608099
  76. The results demonstrate that angiotensin, via type-1 receptors, modulates iron homeostasis in dopaminergic neurons and microglial cells. PMID: 24184051
  77. Acute or repeated exercise reduces the femoral artery responsiveness to angiotensin II. PMID: 23528515
  78. The data here suggest that Ang II is involved in the pro-inflammatory events during HgCl-induced nephropathy, and that this is probably mediated, in part, by Ang II receptors Type 1. PMID: 22822941
  79. Relatively equipressor doses of ANG II and phenylephrine lead to greatly different ambient blood pressure profiles and effects on renal vasculature when assessed in conscious rats. PMID: 23825067
  80. Kamolonol suppresses angiotensin II-induced stress fiber formation and cellular hypertrophy by inhibiting Rho-associated kinase 2 activity PMID: 23891689
  81. Our results show a novel intermediary mechanism that leads to decreased levels of active nNOS in the PVN, involved in subsequent reduction in sympathoinhibition during CHF, offering a new target for the treatment of CHF and other cardiovascular diseases. PMID: 23832698
  82. The study identified the serine phosphorylation (p-Ser) sites induced by PKC-Beta activation or AngII, which inhibits insulin-induced p-Tyr sites on IRS2 and its signals in endothelial cells. PMID: 23775122
  83. Angiotensin II plays a role in glomerular injury in experimental and human diabetic nephropathy via persistent activation of Notch1 and Snail signaling in podocytes resulting in down-regulation of nephrin expression. PMID: 23707238
  84. Angiotensin II regulates long noncoding RNAs implicated in vascular smooth muscle cell production. PMID: 23697773
  85. Increased expression of pulmonary ACE contributes to elevated circulating angiotensin II in gestational protein restriction. PMID: 23365412
  86. Angiotensin II signaling is involved in diabetes mellitus-induced structural changes and oxidative DNA damage in corpus cavernosum. PMID: 23541316
  87. There is a negative feedback function between TGF-beta and ACE2, and the combined inhibition of TGF-beta and activation of the ACE2-Ang-(1-7)-Mas may be useful for treating diabetic renal fibrosis. PMID: 23174757
  88. Angiotensin IV may act through the inhibition of the activity of insulin-regulated aminopeptidase to reduce the degradation of oxytocin at the spinal cord, thereby leading to anti-hyperalgesia in rats with inflammation. PMID: 23142109
  89. these above-mentioned results suggest that Angiotensin II induces down-regulation of miR-30 in cardiomyocytes, which in turn promotes myocardial hypertrophy through excessive autophagy PMID: 23326547
  90. these results indicate that p44/42 MAPK is required for AngII-induced sodium appetite but not thirst or neurohypophysial secretion. PMID: 22913624
  91. Endocrine Ang II controls and communicates with its paracrine counterparts solely through endothelial receptors PMID: 22925777
  92. Exercise training ameliorated the age-induced increase in ANG II vasoconstriction. PMID: 22923503
  93. hese results suggest that RBF, via a unifying mechanism, and tubular function are under strict tonic control of both ANG II and adenosine in both control and diabetic kidneys. PMID: 23283998
  94. The data suggest that these three systems, ANG II, eicosanoids, and endothelin, contribute together and independently to BP control in old female SHR. PMID: 23220478
  95. Both angiotensin-converting enzyme and chymase were needed to convert angiotensin-(1-12) to angiotensin II in the PVN. PMID: 22125313
  96. Angiotensin-II down-regulates cardiac natriuretic peptide receptor-A mediated anti-hypertrophic signaling in experimental rat hearts PMID: 23441479
  97. Forebrain derived ANG II plays a critical role in the salty taste function changes accompanied with acute sodium depletion. PMID: 22846885
  98. Transfection with GPE nanoparticle carrying AGT shRNA can stably lower the blood pressure and improve the atherosclerotic lesions which lead to the delayed development of early atherosclerotic lesions in hypertension rats with concomitant atherosclerosis. PMID: 22977667
  99. this study of the role of ANG II and symathetic nervous system (SNS) in synthesis of extracellular matrix in juvenile rat aorta showed the antagonist action of ANG II and SNS on collagen and elastin synthesis PMID: 22853187
  100. NADPH oxidase 4 (NOX4) is the main catalytic isoform of NADPH oxidase that contributes to ANG II-stimulated oxygen production by medullary thick ascending limbs in rat kidney. PMID: 22875785

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Subcellular Location Secreted
Protein Families Serpin family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

KEGG: rno:24179

STRING: 10116.ENSRNOP00000024917

UniGene: Rn.6319

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