Rat anti-Thyroid-Peroxidase antibody,TPO-Ab ELISA Kit

Code CSB-E11199r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
anti-Thyroid-Peroxidase antibody,TPO-Ab
Abbreviation
TPO Ab
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
5 IU/mL-80 IU/mL
Sensitivity
5 IU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-TPO antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 97    
Range % 86-101    
1:2 Average % 88    
Range % 83-98    
1:4 Average % 95    
Range % 90-100    
1:8 Average % 95    
Range % 89-100    
Recovery
The recovery of rat anti-TPO antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 96 92-99    
EDTA plasma (n=4) 95 90-105    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
IU/ml OD1 OD2 Average      
80 0.171 0.169 0.17      
40 0.285 0.276 0.281      
20 0.495 0.505 0.5      
10 1.037 1.014 1.026      
5 2.032 2.066 2.049      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TPO Ab ELISA Kit was designed for the quantitative measurement of Rat TPO Ab protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 5 IU/mL-80 IU/mL and the sensitivity is 5 IU/mL.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Rat

Sample dilution: 1: 10

Review: I used CSB-E11199r to detect autoimmune thyroiditis in rats, and the dilution ratio was 1: 10. The most important thyroid antibodies for autoimmune thyroiditis are TPOAb and TGAb. We have tried many manufacturers in our laboratory and concluded that the experimental results of Wuhan gorgeous thyroid elisa kit are the most accurate, easy to use and cost-effective!

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