Product Details

Target Name

apolipoprotein B (including Ag(x) antigen)

Alternative Names

Apob ELISA kit; Aa1064 ELISA kit; Ac1-060Apolipoprotein B-100 ELISA kit; Apo B-100) [Cleaved into: Apolipoprotein B-48 ELISA kit; Apo B-48)] ELISA kit

Abbreviation

APOB

Uniprot No.

Q7TMA5

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.313 μg/mL-20 μg/mL

Sensitivity

0.342 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat apolipoprotein B100 (Apo-B100) ELISA Kit is a tool for accurate and reliable measurement of Apob in various sample types including serum, plasma, and tissue homogenates. Apolipoprotein B-100 (Apob) is a protein found in low-density lipoproteins (LDL) and is involved in the transportation of lipids in the blood. Elevated levels of Apob have been linked to various cardiovascular diseases, including atherosclerosis.

This ELISA kit is designed specifically for the quantitative measurement of Apob in Rattus norvegicus (rat) samples. The assay principle is competitive, with a detection range of 0.313 μg/mL-20 μg/mL and a sensitivity of 0.342 μg/mL. The assay time is relatively short, taking only 1-5 hours to obtain accurate and reliable results. The kit comes with a detection wavelength of 450 nm, making it easy to measure and analyze the data.

The Rat apolipoprotein B100 (Apo-B100) ELISA Kit is perfect for researchers in the cardiovascular and signal transduction field who require a sensitive and accurate method to measure Apob levels in rat samples. With its easy-to-follow protocol and high sensitivity, this kit is an excellent tool for the quantification of Apob in various sample types.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

My samples are hemolyzed....could it be any problem with this samples when using the kits?

Thanks for your inquiry. Serious hemolysis is not suitable for testing. You need to re-collect the samples.
If it is slightly hemolyzed, the impact is small and the samples can be used.
The sensitivity of this kit is relatively good, but we still advice you to use dual-wavelength detection to eliminate the impact.

Target Background

Function
(From Uniprot)

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Gene References into Functions

enhanced VLDL TG secretion in the absence of hepatocyte ABCA1 is due to altered intracellular trafficking of apolipoprotein B (apoB), resulting in augmented TG addition to nascent VLDL. PMID: 28694219
Reduced sortilin levels associate with lower VLDL apo B secretion. Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells. PMID: 27495870
Coordinated up-regulation of Mttp, Apob, Hnf1a, Hnf4a, Fasn, Gpam and Dgat (1 and 2) gene expressions may contribute to the increase in circulating and liver concentrations of TAGs after lipectomy in an experimental rat model PMID: 26184122
Overall, results further support the importance of Class I PI3K activity in IDAD, and suggest that loss of this activity is sufficient to cause resistance. PMID: 26616056
KLHL12 plays a critical role in facilitating the endoplasmic reticulum exit and secretion of apoB100-VLDL particles, suggesting that KLHL12 modulation would influence plasma lipid levels. PMID: 24334870
Report abnormal gene/protein expression and phosphorylation of multiple pathways related to inflammatory, insulin signaling and lipogenesis may be important underlying factors in VLDL-apoB100 particles overproduction observed in type 2 diabetes. PMID: 22546076
Doxycycline induced apoA-IV expression in McA-RH7777 cells alters apoB and lipid secretion. PMID: 22257482
JCR:LA-cp rats are used as a model of postprandial lipemia to explore chylomicron metabolism during the onset and development of insulin-resistance by showing impaired apolipoprotein b-48 blood levels. PMID: 16624317
It was concluded that IL-6 favors secretion of apoB-containing lipoproteins by increasing availability of apoB through changes in apob gene transcription. PMID: 20651008
A factor(s) other than microsomal triglyceride transfer protein mediates the formation of the phospholipid-rich primordial apoB:1000-containing initiation complex. PMID: 20181985
the developmental regulation of apoB mRNA editing is an autonomous cytodifferentiation function of small intestine and involves Cdx1 PMID: 12493769
pulse/chase protocols and subcellular fractionation showed apoB100 bulk lipidation, resulting in conversion of LDL/HDL to VLDL, can occur in the ER, but not in the Golgi, in McA RH7777 cells PMID: 12917397
apoB100 lipoproteins exit the endoplasmic reticulum in COPII vesicles, but under conditions favorable for very low density lipoprotein formation final lipid loading occurs post-endoplasmic reticulum PMID: 12960170
apoB mRNA editing is modulated at the level of assembly by alterations in the relative abundance of multiple related RNA-binding auxiliary proteins. PMID: 14570923
increased FA delivery to the liver increases secretion of apoB; oleic acid -induced apoB secretion occurred via mechanisms other than by providing substrate for TG synthesis; and the route of delivery of FA is important for its effects on apoB secretion. PMID: 14970200
Lipid peroxidation and oxidative stress regulate hepatic Apob degradation and VLDL production. PMID: 15124019
the dense apoB-100 intracellular particle is converted to LDL-VLDL 2 by size-dependent lipidation PMID: 15520448
Hepatic Betaine-Homocysteine S-Methyltransferase expression was induced by feeding rats a Met (L-methionine)-restricted betaine-containing diet, and parameters of ApoB metabolism were evaluated. PMID: 16396637
apolipoprotein B secretion, but not lipid, is upregulated by tumor necrosis factor-alpha in rat hepatocyte cultures in the absence of extracellular fatty acids PMID: 17405916
the initial addition of phospholipids to apoB:1000 and initiation of apoB-containing lipoprotein assembly occur independently of MTP lipid transfer activity. PMID: 17690102
apoB secretory control via PERPP/autophagosomes is likely a key component of normal and pathologic regulation of plasma apoB levels, as well as a means for remarkably late-stage quality control of a secreted protein PMID: 18391222
Localization of microsomal triglyceride transfer protein in the Golgi: possible role in the assembly of chylomicrons PMID: 11830580

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Subcellular Location

Cytoplasm. Secreted. Lipid droplet.

Tissue Specificity

Detected in intestine and liver (at protein level).

Database Links

KEGG: rno:54225

STRING: 10116.ENSRNOP00000039779

UniGene: Rn.33815

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Rat apolipoprotein B100 (Apo-B100) ELISA Kit

Product Details

Citations

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Customer Reviews and Q&A

Target Background