Recombinant Rat Apolipoprotein B-100 (Apob), partial

Code CSB-YP766491RA
MSDS
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Source Yeast
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Code CSB-EP766491RA
MSDS
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Source E.coli
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Code CSB-EP766491RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP766491RA
MSDS
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Source Baculovirus
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Code CSB-MP766491RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Apob
Uniprot No.
Alternative Names
Apob; Aa1064; Ac1-060Apolipoprotein B-100; Apo B-100) [Cleaved into: Apolipoprotein B-48; Apo B-48)]
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Gene References into Functions
  1. enhanced VLDL TG secretion in the absence of hepatocyte ABCA1 is due to altered intracellular trafficking of apolipoprotein B (apoB), resulting in augmented TG addition to nascent VLDL. PMID: 28694219
  2. Reduced sortilin levels associate with lower VLDL apo B secretion. Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells. PMID: 27495870
  3. Coordinated up-regulation of Mttp, Apob, Hnf1a, Hnf4a, Fasn, Gpam and Dgat (1 and 2) gene expressions may contribute to the increase in circulating and liver concentrations of TAGs after lipectomy in an experimental rat model PMID: 26184122
  4. Overall, results further support the importance of Class I PI3K activity in IDAD, and suggest that loss of this activity is sufficient to cause resistance. PMID: 26616056
  5. KLHL12 plays a critical role in facilitating the endoplasmic reticulum exit and secretion of apoB100-VLDL particles, suggesting that KLHL12 modulation would influence plasma lipid levels. PMID: 24334870
  6. Report abnormal gene/protein expression and phosphorylation of multiple pathways related to inflammatory, insulin signaling and lipogenesis may be important underlying factors in VLDL-apoB100 particles overproduction observed in type 2 diabetes. PMID: 22546076
  7. Doxycycline induced apoA-IV expression in McA-RH7777 cells alters apoB and lipid secretion. PMID: 22257482
  8. JCR:LA-cp rats are used as a model of postprandial lipemia to explore chylomicron metabolism during the onset and development of insulin-resistance by showing impaired apolipoprotein b-48 blood levels. PMID: 16624317
  9. It was concluded that IL-6 favors secretion of apoB-containing lipoproteins by increasing availability of apoB through changes in apob gene transcription. PMID: 20651008
  10. A factor(s) other than microsomal triglyceride transfer protein mediates the formation of the phospholipid-rich primordial apoB:1000-containing initiation complex. PMID: 20181985
  11. the developmental regulation of apoB mRNA editing is an autonomous cytodifferentiation function of small intestine and involves Cdx1 PMID: 12493769
  12. pulse/chase protocols and subcellular fractionation showed apoB100 bulk lipidation, resulting in conversion of LDL/HDL to VLDL, can occur in the ER, but not in the Golgi, in McA RH7777 cells PMID: 12917397
  13. apoB100 lipoproteins exit the endoplasmic reticulum in COPII vesicles, but under conditions favorable for very low density lipoprotein formation final lipid loading occurs post-endoplasmic reticulum PMID: 12960170
  14. apoB mRNA editing is modulated at the level of assembly by alterations in the relative abundance of multiple related RNA-binding auxiliary proteins. PMID: 14570923
  15. increased FA delivery to the liver increases secretion of apoB; oleic acid -induced apoB secretion occurred via mechanisms other than by providing substrate for TG synthesis; and the route of delivery of FA is important for its effects on apoB secretion. PMID: 14970200
  16. Lipid peroxidation and oxidative stress regulate hepatic Apob degradation and VLDL production. PMID: 15124019
  17. the dense apoB-100 intracellular particle is converted to LDL-VLDL 2 by size-dependent lipidation PMID: 15520448
  18. Hepatic Betaine-Homocysteine S-Methyltransferase expression was induced by feeding rats a Met (L-methionine)-restricted betaine-containing diet, and parameters of ApoB metabolism were evaluated. PMID: 16396637
  19. apolipoprotein B secretion, but not lipid, is upregulated by tumor necrosis factor-alpha in rat hepatocyte cultures in the absence of extracellular fatty acids PMID: 17405916
  20. the initial addition of phospholipids to apoB:1000 and initiation of apoB-containing lipoprotein assembly occur independently of MTP lipid transfer activity. PMID: 17690102
  21. apoB secretory control via PERPP/autophagosomes is likely a key component of normal and pathologic regulation of plasma apoB levels, as well as a means for remarkably late-stage quality control of a secreted protein PMID: 18391222
  22. Localization of microsomal triglyceride transfer protein in the Golgi: possible role in the assembly of chylomicrons PMID: 11830580

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Subcellular Location
Cytoplasm. Secreted. Lipid droplet.
Tissue Specificity
Detected in intestine and liver (at protein level).
Database Links
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