Rat calmodulin,CAM ELISA Kit

Code CSB-E09753r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P0DP29
Lead Time 3-5 working days
Abbreviation CALM1
Target Name calmodulin 1 (phosphorylase kinase, delta)
Alias CAMI, DD132, PHKD, calmodulin 1|phosphorylase kinase, delta subunit, CALM1 CALM CAM CAM1, CALM2 CAM2 CAMB, CALM3 CALML2 CAM3 CAMC CAMIII
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %88
Range %85-91
1:10Average %96
Range %94-98
1:20Average %89
Range %87-90
1:40Average %104
Range %101-107
Recovery
The recovery of rat CAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9995-102
EDTA plasma (n=4)104101-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.819 2.836 2.828 2.632
5002.181 2.101 2.141 1.945
2501.494 1.475 1.485 1.289
1250.919 0.935 0.927 0.731
62.50.588 0.598 0.593 0.397
31.20.402 0.432 0.417 0.221
15.60.287 0.275 0.281 0.085
00.195 0.197 0.196
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Function Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.
Subcellular Location Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, spindle pole
Protein Families Calmodulin family
Database Links

KEGG: rno:24242

UniGene: Rn.129719

Pathway Excitatory synapse pathway

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