Rat calmodulin,CAM ELISA Kit

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Product Details

Target Name

calmodulin 1 (phosphorylase kinase, delta)

Alternative Names

CAMI, DD132, PHKD, calmodulin 1|phosphorylase kinase, delta subunit, CALM1 CALM CAM CAM1, CALM2 CAM2 CAMB, CALM3 CALML2 CAM3 CAMC CAMIII

Abbreviation

CALM1

Uniprot No.

P0DP29

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	88
	Range %	85-91
1:10	Average %	96
	Range %	94-98
1:20	Average %	89
	Range %	87-90
1:40	Average %	104
	Range %	101-107

Recovery

The recovery of rat CAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-102
EDTA plasma (n=4)	104	101-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.819	2.836	2.828	2.632
500	2.181	2.101	2.141	1.945
250	1.494	1.475	1.485	1.289
125	0.919	0.935	0.927	0.731
62.5	0.588	0.598	0.593	0.397
31.2	0.402	0.432	0.417	0.221
15.6	0.287	0.275	0.281	0.085
0	0.195	0.197	0.196

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

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Target Data

Function	Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Mediates calcium-dependent inactivation of CACNA1C. Positively regulates calcium-activated potassium channel activity of KCNN2.
Subcellular Location	Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, spindle pole
Protein Families	Calmodulin family
Database Links	KEGG: rno:24242 UniGene: Rn.129719

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Check Code：

Code	CSB-E09753r
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.