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Rat coagulation factor Ⅹ,FⅩ ELISA Kit

Citations (1) Protocol MSDS
Code CSB-E08441r
Size 96T,5×96T,10×96T
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Product Details

Target Name
coagulation factor X
Alternative Names
F10Coagulation factor X ELISA Kit; EC 3.4.21.6 ELISA Kit; Stuart factor) [Cleaved into: Factor X light chain; Factor X heavy chain; Activated factor Xa heavy chain] ELISA Kit
Abbreviation
F10
Uniprot No.
Q63207
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Blood Coagulation
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FⅩ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　

Sample

Serum(n=4)

1:1000

Average %

97

Range %

95-102

1:2000

Average %

98

Range %

91-105

1:4000

Average %

106

Range %

102-109

1:8000

Average %

92

Range %

86-98

Recovery

The recovery of rat FⅩ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

95

89-98

EDTA plasma (n=4)

96

92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

100

1.959

1.914

1.936

1.785

50

1.679

1.657

1.668

1.517

25

1.300

1.338

1.319

1.168

12.5

0.993

0.969

0.981

0.830

6.25

0.678

0.651

0.664

0.513

3.12

0.457

0.443

0.450

0.299

1.56

0.377

0.384

0.380

0.229

0

0.148

0.155

0.151

　

ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat F10 ELISA Kit was designed for the quantitative measurement of Rat F10 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Citations

Related Products

F10 Proteins

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Gene References into Functions
  1. Factor Xa inhibitor edoxaban at or higher doses than needed for an antithrombotic effect sustained the circulating Gla-osteocalcin level. PMID: 22999414
  2. Report differential effects of TAK-442, a novel orally active direct factor Xa inhibitor, and ximelagatran, a thrombin inhibitor, on factor V-mediated feedback on coagulation cascade and bleeding. PMID: 20664909
  3. Factor Xa has a role in inducing mitogenesis of vascular smooth muscle cells by autocrine production of epiregulin PMID: 14570897
  4. The changes in levels of blood coagulation factor X in folic acid deficient rats are reversed by treatment with folic acid. PMID: 16046705
Subcellular Location
Secreted.
Protein Families
Peptidase S1 family
Tissue Specificity
Plasma; synthesized in the liver.
Database Links
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Related pathways
Complement and coagulation cascades
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