Rat eosinophil cationic protein,ECP ELISA Kit

Code CSB-E11238r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
ribonuclease, RNase A family, 3 (eosinophil cationic protein)
Alternative Names
Rnase3 ELISA Kit; Ear11 ELISA Kit; Rns3 ELISA Kit; Eosinophil cationic protein ELISA Kit; ECP ELISA Kit; EC 3.1.27.- ELISA Kit; Eosinophil secondary granule ribonuclease 11 ELISA Kit; EAR-11 ELISA Kit; Ribonuclease 3 ELISA Kit; RNase 3 ELISA Kit
Abbreviation
RNASE3
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-100
1:2 Average % 101
Range % 96-107
1:4 Average % 92
Range % 88-96
1:8 Average % 89
Range % 84-93
Recovery
The recovery of rat ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-98
EDTA plasma (n=4) 87 82-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.651 2.529 2.590 2.382
2000 2.004 1.903 1.954 1.746
1000 1.310 1.385 1.348 1.140
500 0.829 0.851 0.840 0.632
250 0.492 0.476 0.484 0.276
125 0.347 0.337 0.342 0.134
62.5 0.285 0.294 0.290 0.082
0 0.203 0.212 0.208  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat RNASE3 ELISA Kit was designed for the quantitative measurement of Rat RNASE3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function
(From Uniprot)
Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities.
Subcellular Location
Cytoplasmic granule.
Protein Families
Pancreatic ribonuclease family
Database Links
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