Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

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Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

Citations (8) Protocol

Code	CSB-E14981r
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations (8)
Customer Reviews and Q&A
Target Background

Target Name

superoxide dismutase 3, extracellular

Alternative Names

Sod3 ELISA Kit; Sod-3 ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; EC-SOD ELISA Kit; EC 1.15.1.1 ELISA Kit; Superoxide dismutase B ELISA Kit

Abbreviation

SOD3

Uniprot No.

Q08420

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Cu/Zn-SOD/SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	93
	Range %	89-95
1:400	Average %	104
	Range %	100-107
1:800	Average %	95
	Range %	90-97
1:1600	Average %	98
	Range %	91-101

Recovery

The recovery of rat Cu/Zn-SOD/SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	95	92-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.644	2.588	2.616	2.461
500	2.264	2.344	2.304	2.149
250	1.650	1.632	1.641	1.486
125	1.044	1.028	1.036	0.881
62.5	0.624	0.636	0.630	0.475
31.2	0.383	0.368	0.376	0.221
15.6	0.248	0.239	0.244	0.089
0	0.156	0.154	0.155

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Role of ADMA/DDAH-1 and iNOS/eNOS signaling in the gastroprotective effect of tadalafil against indomethacin-induced gastric injury YT Mohamed,Biomedicine & Pharmacotherapy,2022
Consumption of Cashew (Anacardium occidentale L.) Nuts Counteracts Oxidative Stress and Tissue Inflammation in Mild Hyperhomocysteinemia in Rats R D'Amico,Nutrients,2022
Potential Toxic Effects of Exposure to Titanium Silicon Oxide Nanoparticles in Male Rats S Ghareeb,Toxicology in vitro,2022
Hydroxycitrate prevents calcium oxalate crystallization and kidney injury in a nephrolithiasis rat model Bowei Yang,Urolithiasis,2021
Expression of nAChRα7 receptor in model rats with Parkinson's disease dementia Y Pan,Biotechnology & Biotechnological Equipment,2020
Effect of Light-Dark Cycle Misalignment on the Hypothalamic-Pituitary-Gonadal Axis, Testicular Oxidative Stress, and Expression of Clock Genes in Adult Male Rats A Moustafa,International journal of endocrinology,2020
Hematobiochemical Disturbances and Oxidative Stress After Subacute Manganese Chloride Exposure and Potential Protective Effects of Ebselen in Rats Hager Tarek H.et al,Biological Trace Element Research,2018
A putative Chondroprotective role for IL-1B and MPO in herbal treatment of experimental osteoarthritis Aborehab NM.et al,BMC Complement Altern Med,2017

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Function (From Uniprot)	Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Gene References into Functions	Data show that aging is associated with reduction in superoxide dismutase (SOD) Cu/Zn-SOD protein expression and total SOD enzymatic activity in mesenteric lymphatic vessel (MLV). PMID: 22540739 The impact of SOD3 on Akt and Erk1/2 cell survival pathways in rat hind limb injury model, is reported. PMID: 21909393 ER stress leads to the down-regulation of claudin-5 among tight junction proteins and may induce the elevation of endothelial permeability and leakage of EC-SOD into the vitreous body. PMID: 21736484 These data are important for understanding the pathogenesis of vascular diseases in which the synthesis of endothelial NO is decreased or extracellular O2- production exceeds the functional capacity of SOD3. PMID: 20033309 Furin proteolytically processes the heparin-binding region of extracellular superoxide dismutase PMID: 11861638 Levels are not significantly altered in the rat prostate during aging and thus is unlikely to be an important factor in the evolution of epithelial cell hyperplasia PMID: 16372329 EcSOD, CuZnSOD, catalase, and MMP-2 mRNA expression did not statistically vary between aortic aneurysm and normal tissue PMID: 17196988 SOD3 minimizes O2- levels preserving NO availability at resting conditions. SOD3 promotes NO mediated vasodilatation by scavenging O2- and basal SOD3 levels is able to inactivate O2- produced by 100% oxygen breathing preserving vasodilator effect of NO. PMID: 19198181 SOD3 has a selective anti-inflammatory role in ischemic damages preventing the migration of reactive oxygen producing monocyte/macrophages PMID: 19495415 Show More Hide All
Subcellular Location	Secreted, extracellular space. Golgi apparatus, trans-Golgi network.
Protein Families	Cu-Zn superoxide dismutase family
Database Links	KEGG: rno:25352 STRING: 10116.ENSRNOP00000005155 UniGene: Rn.10358

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Related pathways

Cell ageing and metabolism

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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