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Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

Instructions
Code CSB-E14981r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name superoxide dismutase 3, extracellular
Alternative Names Sod3 ELISA Kit; Sod-3 ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; EC-SOD ELISA Kit; EC 1.15.1.1 ELISA Kit; Superoxide dismutase B ELISA Kit
Abbreviation SOD3
Uniprot No. Q08420
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Cu/Zn-SOD/SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:200Average %93
Range %89-95
1:400Average %104
Range %100-107
1:800Average %95
Range %90-97
1:1600Average %98
Range %91-101
Recovery
The recovery of rat Cu/Zn-SOD/SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9592-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.644 2.588 2.616 2.461
5002.264 2.344 2.304 2.149
2501.650 1.632 1.641 1.486
1251.044 1.028 1.036 0.881
62.50.624 0.636 0.630 0.475
31.20.383 0.368 0.376 0.221
15.60.248 0.239 0.244 0.089
00.156 0.154 0.155 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)		 Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Gene References into Functions
  1. Data show that aging is associated with reduction in superoxide dismutase (SOD) Cu/Zn-SOD protein expression and total SOD enzymatic activity in mesenteric lymphatic vessel (MLV). PMID: 22540739
  2. The impact of SOD3 on Akt and Erk1/2 cell survival pathways in rat hind limb injury model, is reported. PMID: 21909393
  3. ER stress leads to the down-regulation of claudin-5 among tight junction proteins and may induce the elevation of endothelial permeability and leakage of EC-SOD into the vitreous body. PMID: 21736484
  4. These data are important for understanding the pathogenesis of vascular diseases in which the synthesis of endothelial NO is decreased or extracellular O2- production exceeds the functional capacity of SOD3. PMID: 20033309
  5. Furin proteolytically processes the heparin-binding region of extracellular superoxide dismutase PMID: 11861638
  6. Levels are not significantly altered in the rat prostate during aging and thus is unlikely to be an important factor in the evolution of epithelial cell hyperplasia PMID: 16372329
  7. EcSOD, CuZnSOD, catalase, and MMP-2 mRNA expression did not statistically vary between aortic aneurysm and normal tissue PMID: 17196988
  8. SOD3 minimizes O2- levels preserving NO availability at resting conditions. SOD3 promotes NO mediated vasodilatation by scavenging O2- and basal SOD3 levels is able to inactivate O2- produced by 100% oxygen breathing preserving vasodilator effect of NO. PMID: 19198181
  9. SOD3 has a selective anti-inflammatory role in ischemic damages preventing the migration of reactive oxygen producing monocyte/macrophages PMID: 19495415

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Subcellular Location Secreted, extracellular space
Protein Families Cu-Zn superoxide dismutase family
Database Links

KEGG: rno:25352

STRING: 10116.ENSRNOP00000005155

UniGene: Rn.10358
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