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Rat glycosaminoglycan/mucopolysaccharide ELISA kit

Citations (1) Protocol
Code CSB-EQ027297RA
Size 96T,5×96T,10×96T
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Product Details

Target Name
glycosaminoglycan/mucopolysaccharide
Abbreviation
GAGs
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, urine
Detection Range
6.25 ng/mL-100 ng/mL
Sensitivity
3.125 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat glycosaminoglycan in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:100Average %91
Range %86-97
1:200Average %98
Range %93-104
1:400Average %85
Range %81-88
1:800Average %88
Range %83-94
Recovery
The recovery of rat glycosaminoglycan spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-94
EDTA plasma (n=4)9288-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
1000.081 0.086 0.084
500.195 0.201 0.198
250.490 0.510 0.500
12.50.925 0.902 0.914
6.251.688 1.705 1.697
02.587 2.612 2.600
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat GAGs ELISA Kit was designed for the quantitative measurement of Rat GAGs protein in serum, plasma, urine. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-100 ng/mL and the sensitivity is 3.125 ng/mL .

Citations

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