Product Details

Target Name

growth hormone releasing hormone

Alternative Names

Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit

Abbreviation

GHRH

Uniprot No.

P09916

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	92
	Range %	85-99
1:4	Average %	90
	Range %	88-94
1:8	Average %	101
	Range %	96-105

Recovery

The recovery of rat GHRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-95
EDTA plasma (n=4)	106	103-111

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.088	2.252	2.170	2.080
100	1.585	1.676	1.631	1.541
50	1.163	1.104	1.134	1.044
25	0.831	0.765	0.798	0.708
12.5	0.550	0.524	0.537	0.447
6.25	0.344	0.345	0.345	0.255
3.12	0.182	0.183	0.183	0.093
0	0.090	0.089	0.090

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat GHRH ELISA Kit was designed for the quantitative measurement of Rat GHRH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

Target Background

Function
(From Uniprot)

GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.

Gene References into Functions

GHRH agonistic analogs, JI-34 and MR-356 alleviated radiation-induced damage of cardiac myocytes. PMID: 27480202
findings support the hypothesis that antagonizing the signaling of GHRH in T1D may improve GLP-1 function in the small intestine, which, in turn, diminishes TRL and reduces renal and vascular complications PMID: 26831066
JI-38 had shown promising results in multiple fields including cardiology, diabetes and wound healing, our new, more potent, GHRH agonists should manifest additional efficacy for possible medical applications. PMID: 24373935
GHRH protects the heart from ischemia/reperfusion injury through receptor-mediated mechanisms, leading to activation of RISK and SAFE pathways, which converge on mitochondria and possibly on AMP-activated protein kinase. PMID: 23417421
We present evidence that growth retardation in upper airway obstruction is associated with a reduction in hypothalamic GHRH content. PMID: 21406516
Growth hormone expression rises in pituitary gland during normal pregnancy, while its receptor mRNA level in hypothalamus decreases. PMID: 20814072
GABAergic neurons in the median preoptic nucleus and ventrolateral preoptic nucleus are potential targets of the sleep-regulatory actions of GHRH. PMID: 19889861
binding of [125I-Tyr(10)]hGHRH NH(2) to medullary homogenates was specific, time-dependent, reversible and saturable (K(d): 28 nM; B(max): 30 fmol/mg prot.). PMID: 11814616
direct interactions between the growth hormone releasing hormone and growth hormone secretagogue receptors may explain the observed effects of of ghrelin and growth hormone secretagogues on signal transduction PMID: 12446584
Review. Growth hormone-releasing hormone (GHRH) is a hypothalamic hormone that is essential for normal expansion of the somatotrope lineage during pituitary development. PMID: 12511850
Changes in ghrelin and ghrelin receptor expression according to feeding status. PMID: 12876464
Growth hormone releasing hormone (GHRH) is involved in the transcriptional regulation of the GH gene. PMID: 14664705
results suggest that growth hormone secretagogue receptor is involved in the up-regulation of growth hormone releasing hormone and neuropeptide Y expression PMID: 16728494
RFRP-3 infusion elicited a significant increase in growth hormone-releasing hormone mRNA and plasma growth hormone levels compared to control rats. PMID: 18628614
Growth hormone-releasing hormone promotes survival of cardiac myocytes in vitro and protects against ischaemia-reperfusion injury in rat heart. PMID: 19293247
Postsynaptic localization of sst(1) receptors and synaptic connectivity may be an important mechanism by which sst(1) mediates SRIF's cyclical effects on ARC GHRH neurons to generate the ultradian rhythm of GH secretion. PMID: 19553459

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Subcellular Location

Secreted.

Protein Families

Glucagon family

Database Links

KEGG: rno:29446

STRING: 10116.ENSRNOP00000010298

UniGene: Rn.10693

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Code	CSB-E06878r
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Rat growth hormone releasing hormone,GHRH ELISA Kit

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Target Background