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Rat guanylate cyclase activator 2B (GUCA2B) ELISA kit

Citations (1) Protocol MSDS
Code CSB-EL010048RA
Size 96T,5×96T,10×96T
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Product Details

Target Name
guanylate cyclase activator 2B (uroguanylin)
Alternative Names
Guca2b ELISA Kit; Guanylate cyclase activator 2B [Cleaved into: Uroguanylin ELISA Kit; UGN)] ELISA Kit
Abbreviation
GUCA2B
Uniprot No.
P70668
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 97    
Range % 88-102    
1:2 Average % 92    
Range % 85-96    
1:4 Average % 90    
Range % 85-94    
1:8 Average % 91    
Range % 84-95    
Recovery
The recovery of rat GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 88 81-92    
EDTA plasma (n=4) 104 92-107    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
500 1.997 1.943 1.970 1.831    
250 1.508 1.476 1.492 1.353    
125 1.002 0.987 0.995 0.856    
62.5 0.572 0.565 0.569 0.430    
31.25 0.428 0.420 0.424 0.285    
15.6 0.289 0.276 0.283 0.144    
7.8 0.204 0.198 0.201 0.062    
0 0.141 0.137 0.139 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat GUCA2B ELISA Kit was designed for the quantitative measurement of Rat GUCA2B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Citations

Related Products

Guca2b Proteins

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.
Gene References into Functions
  1. our data suggest that the inhibitory effect of UGN on NHE3 transport activity in proximal tubule is mediated by activation of both cGMP/PKG and cAMP/PKA signaling pathways PMID: 22952280
  2. Uroguanylin is delivered to the kidney as an unprocessed propeptide. PMID: 18499760
  3. To respond to proUGn released from the gut, any target organ must use a local mechanism for production of active peptide. PMID: 18499761
Subcellular Location
Secreted.
Protein Families
Guanylin family
Tissue Specificity
Expressed not only in the gastrointestinal tract but also in the lung, pancreas and kidney.
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