Rat inhibin B,INH-B ELISA Kit

Instructions
Code CSB-E08150r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name inhibin B,INH-B
Alternative Names N/A
Abbreviation INH-B
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1 pg/mL-200 pg/mL
Sensitivity 0.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-106
1:2Average %98
Range %90-105
1:4Average %94
Range %89-99
1:8Average %96
Range %90-100
Recovery
The recovery of rat INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9687-104
EDTA plasma (n=4)9791-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
2000.240 0.246 0.243
500.291 0.305 0.298
160.421 0.432 0.427
40.722 0.697 0.710
11.117 1.146 1.132
02.188 2.164 2.176
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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