Mouse inhibin B,INH-B ELISA Kit

Instructions
Code CSB-E08151m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name inhibin B
Alternative Names EDF, FRP, FSH-releasing protein|Inhibin, beta-1|OTTHUMP00000207260|erythroid differentiation factor|follicle-stimulating hormone-releasing protein|inhibin beta A|inhibin, beta A (activin A, activin
Abbreviation INH-B
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 1 pg/mL-200 pg/mL
Sensitivity 0.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 82-94  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 98  
Range % 92-103  
1:8 Average % 87  
Range % 81-92  
Recovery
The recovery of mouse INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 91-102  
EDTA plasma (n=4) 102 98-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.183 0.181 0.182    
50 0.342 0.338 0.340    
16 0.564 0.552 0.558    
4 0.896 0.838 0.867    
1 1.484 1.412 1.448    
0 1.824 1.984 1.904    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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