Mouse inhibin B,INH-B ELISA Kit

Code CSB-E08151m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation INH-B
Target Name inhibin B
Alias EDF, FRP, FSH-releasing protein|Inhibin, beta-1|OTTHUMP00000207260|erythroid differentiation factor|follicle-stimulating hormone-releasing protein|inhibin beta A|inhibin, beta A (activin A, activin
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 1 pg/mL-200 pg/mL
Sensitivity 0.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.
HGNC 6066
RGD 62074
MGI 96570
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 82-94  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 98  
Range % 92-103  
1:8 Average % 87  
Range % 81-92  
Recovery
The recovery of mouse INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 91-102  
EDTA plasma (n=4) 102 98-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.183 0.181 0.182    
50 0.342 0.338 0.340    
16 0.564 0.552 0.558    
4 0.896 0.838 0.867    
1 1.484 1.412 1.448    
0 1.824 1.984 1.904    
References
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