Human inhibin B,INH-B ELISA Kit

Code CSB-E08149h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
inhibin B,INH-B
Alternative Names
N/A
Abbreviation
INH-B
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4 pg/mL-800 pg/mL
Sensitivity
2 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 94-102  
1:2 Average % 93  
Range % 89-97  
1:4 Average % 99  
Range % 96-101  
1:8 Average % 92  
Range % 86-96  
Recovery
The recovery of human INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 80-88  
EDTA plasma (n=4) 96 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
800 0.223 0.224 0.224    
200 0.355 0.362 0.359    
60 0.556 0.568 0.562    
16 0.817 0.803 0.810    
4 1.374 1.275 1.325    
0 2.508 2.404 2.456    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human INH-B ELISA Kit was designed for the quantitative measurement of Human INH-B protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 4 pg/mL-800 pg/mL and the sensitivity is 2 pg/mL.

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