Rat leukemia inhibitory factor,LIF ELISA Kit

Code CSB-E07432r
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P17777
Lead Time 3-5 working days
Abbreviation LIF
Target Name leukemia inhibitory factor (cholinergic differentiation factor)
Alias CDF, DIA, HILDA, D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation stimulating factor
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
HGNC 6596
RGD 620865
MGI 96787
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %84-95
1:2Average %93
Range %89-97
1:4Average %99
Range %93-104
1:8Average %90
Range %86-98
Recovery
The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-92
EDTA plasma (n=4)9589-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.249 2.174 2.212 2.039
101.583 1.561 1.572 1.399
51.012 0.952 0.982 0.809
2.50.599 0.583 0.591 0.418
1.250.446 0.416 0.431 0.258
0.6250.320 0.299 0.310 0.137
0.3120.258 0.245 0.252 0.079
00.177 0.169 0.173  
References
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Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subcellular Location Secreted
Protein Families LIF/OSM family
Database Links

STRING: 10116.ENSRNOP00000009313

UniGene: Rn.44379

Pathway Jak-STAT signaling pathway
TNF signaling pathway
Signaling pathways regulating pluripotency of stem cells
Excitatory synapse pathway

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