Rat leukemia inhibitory factor,LIF ELISA Kit

Code CSB-E07432r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
leukemia inhibitory factor (cholinergic differentiation factor)
Alternative Names
LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
Abbreviation
LIF
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %84-95
1:2Average %93
Range %89-97
1:4Average %99
Range %93-104
1:8Average %90
Range %86-98
Recovery
The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-92
EDTA plasma (n=4)9589-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.249 2.174 2.212 2.039
101.583 1.561 1.572 1.399
51.012 0.952 0.982 0.809
2.50.599 0.583 0.591 0.418
1.250.446 0.416 0.431 0.258
0.6250.320 0.299 0.310 0.137
0.3120.258 0.245 0.252 0.079
00.177 0.169 0.173  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat LIF ELISA Kit was designed for the quantitative measurement of Rat LIF protein in serum, plasma, bronchoalveolar lavage fluid, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Target Background

Function
(From Uniprot)
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subcellular Location
Secreted.
Protein Families
LIF/OSM family
Database Links
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