Rat leukemia inhibitory factor,LIF ELISA Kit

Code CSB-E07432r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leukemia inhibitory factor (cholinergic differentiation factor)
Alternative Names LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
Abbreviation LIF
Uniprot No. P17777
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %89
Range %84-95
1:2Average %93
Range %89-97
1:4Average %99
Range %93-104
1:8Average %90
Range %86-98
The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-92
EDTA plasma (n=4)9589-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
202.249 2.174 2.212 2.039
101.583 1.561 1.572 1.399
51.012 0.952 0.982 0.809
2.50.599 0.583 0.591 0.418
1.250.446 0.416 0.431 0.258
0.6250.320 0.299 0.310 0.137
0.3120.258 0.245 0.252 0.079
00.177 0.169 0.173  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subcellular Location Secreted
Protein Families LIF/OSM family
Database Links

STRING: 10116.ENSRNOP00000009313

UniGene: Rn.44379

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