Rat lipoprotein lipase,LPL ELISA Kit

Instructions
Code CSB-E08494r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lipoprotein lipase
Alternative Names Lpl ELISA Kit; Lipoprotein lipase ELISA Kit; LPL ELISA Kit; EC 3.1.1.34 ELISA Kit
Abbreviation LPL
Uniprot No. Q06000
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 93  
Range % 87-96  
1:400 Average % 102  
Range % 97-105  
1:800 Average % 97  
Range % 92-101  
1:1600 Average % 98  
Range % 93-101  
Recovery
The recovery of rat LPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 84-91  
EDTA plasma (n=4) 99 94-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.538 2.512 2.525 2.413  
250 2.014 1.977 1.996 1.884  
125 1.493 1.464 1.479 1.367  
62.5 0.801 0.817 0.809 0.697  
31.2 0.483 0.466 0.475 0.363  
15.6 0.281 0.272 0.277 0.165  
7.8 0.194 0.188 0.191 0.079  
0 0.113 0.111 0.112    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
Gene References into Functions
  1. The results of the present study indicated that maternal undernutrition during pregnancy may lead to an increase in hepatic triglycerides, via alterations in the transcriptional and epigenetic regulation of the LPL gene. PMID: 27035287
  2. Cardiomyocyte VEGF activates EC Notch signaling, facilitating GPIHBP1-mediated translocation of LPL across EC and regulating LPL-derived fatty acid delivery to the cardiomyocytes. PMID: 26586663
  3. the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue. PMID: 25589507
  4. Data indicate that oxidation of oleic acid was reduced by 50% in lipoprotein lipase LPL-knock-down (LPL-KD) cells compared to wild-type (WT) cells. PMID: 25931001
  5. Activated cyclin-dependent kinase 5 promotes microglial phagocytosis of fibrillar beta-amyloid by up-regulating lipoprotein lipase expression PMID: 23816988
  6. Suggest that the heparanase-lipoprotein lipase-VEGF axis amplifies fatty acid delivery, a rapid and adaptive mechanism that is geared to overcome the loss of glucose consumption by the diabetic heart. PMID: 24115032
  7. The plasticity of the LPL system is severely blunted or completely lost in insulin resistant rats. PMID: 23176178
  8. active heparanase released lipoprotein lipase from the myocyte surface PMID: 23471235
  9. Data suggest that endothelial lipoprotein lipase (LPL) levels in the heart are altered to maintain FA flux and may exploit angiopoietin-like protein-4 (ANGPTL4). PMID: 22226882
  10. Report a simple procedure for measuring lipoprotein lipase activity. PMID: 22870821
  11. In acute hyperglycemia and moderate diabetes, exaggerated LPL processing to dimeric, catalytically active enzyme increases coronary LPL, delivering more FA to the heart when glucose utilization is compromised. PMID: 21646389
  12. This study showed that adiponectin mediates actin cytoskeleton remodeling to translocate lipoprotein lipase and allow subsequent activation. PMID: 21147877
  13. When compared with obesity-resistant rats, the gene expression of LPL was higher in obesity-prone rats. PMID: 17712951
  14. The over-expression of LPL in the hippocampus may play an important role in the oxidative stress mechanisms following status epilepticus (SE) by regulating the uptake of vitamin E. PMID: 20497648
  15. Down-regulation of adipose tissue lipoprotein lipase during fasting requires that a gene, separate from the lipase gene, is switched on PMID: 11809775
  16. An HFS diet induces changes in LPL and VLDL-R in a manner which favors shunting of dietary fat from skeletal muscle to adipose tissue. PMID: 11882325
  17. characterized changes in LPL activity in adipose tissue and muscles before and during the early phase of obesity development in the JCR:LA-cp rat PMID: 11896485
  18. STZ-induced diabetes causes a decrease in LPL activity in the sciatic nerve that is reversible with insulin treatment PMID: 11897675
  19. insulin deficiency impairs the expression of LPL in mammary glands, revealing the role of insulin as a modulator of the enzyme at the mRNA expression level PMID: 11900280
  20. The hormonal responses of LPL activity and lipolysis in adipose tissue differed depending on the stage of the estrous cycle. PMID: 12032743
  21. Although Lpl increases arterial wall accumulation of triglyceride-rich particles(TGRPs), it also reduces the penetration of TGRPs into the arterial wall--a novel antiatherogenic property. PMID: 12482838
  22. PPAR-gamma activation mediates adipose depot-specific effects on gene expression and activity of this enzyme. PMID: 12540599
  23. the nutritional regulation of LPL in adipose tissue determines the activity state of extracellular LPL. PMID: 12551943
  24. the striking sensitivity of muscle lipoprotein lipase to inactivity and low-intensity contractile activity may indicate why inactivity is a risk factor for metabolic diseases PMID: 12815182
  25. Rapid "metabolic switching" occurs through Lpl occupation of endothelial binding-sites. PMID: 12967632
  26. AMPK-mediated recruitment of LPL to the coronary lumen could represent an immediate compensatory response by the heart to guarantee fatty acid supply. PMID: 15328075
  27. The decrease of LPL mass and activity that occurs when explants of rat adipose tissue are incubated is due to proteolysis of extracellular LPL. The effect of inhibitors indicates, but does not prove, that the degradation is mediated by MMPs. PMID: 15670333
  28. Lysophosphatidic acid induced increases in cardiac lipoprotein lipase occurred via posttranscriptional mechanisms and processes. PMID: 15681706
  29. Upregulated by rosiglitazone PMID: 15887043
  30. Chronic stress decreased LPL in mesenteric and epididymal white adipose tissue(WAT). Acute stress decreased LPL only in retroperitoneal WAT and increased LPL in preheparin plasma active LPL. PMID: 15947029
  31. The finding that nephrotic serum alters lipoprotein lipase (LpL) binding to rat aortic endothelial cells suggests LpL depletion results from decreased binding rather than defective delivery. PMID: 16807550
  32. Angiopoietin-like protein 4 is a fasting-induced controller of lipoprotein lipase in adipose tissue, acting extracellularly on the native conformation in an unusual fashion, like an unfolding molecular chaperone PMID: 17088546
  33. Accelerated triglyceride clearance was associated with increased Lpl activity, mostly in brown adipose tissue. PMID: 17170230
  34. the release from endothelium to the bloodstream may constitute a fast nonexplored mechanism of tissue LPL activity regulation, involved in the lipid energy-substrate redistribution between tissues needed to prepare the "fight-or-flight" respo PMID: 17259660
  35. hormonal and nutritional induction of liver PPAR-alpha expression around birth and its maintained elevated level throughout suckling is responsible for the induction of liver LPL-expression and activity during suckling PMID: 17451160
  36. Suggest the involvement of brain phosphatidylinositol-specific PLC and diacylglycerol lipase in the centrally administered histamine-induced activation of the adrenomedullary outflow in rats. PMID: 17628524
  37. oxidized fat impairs normal metabolic adaptations during lactation, which promote the utilization of metabolic substrates by the mammary gland for the synthesis of milk PMID: 17709442
  38. Administration of resistin to adipocytes mimicked the effects of GIP on the PKB/LKB1/AMPK/LPL pathway: increasing phosphorylation of PKB, reducing levels of phosphorylated LKB1 and AMPK, and increasing LPL activity. PMID: 17890220
  39. Recruitment of LPL to the cardiomyocyte surface represents an immediate compensatory response by the heart to guarantee fatty acid supply when glucose utilization is compromised. PMID: 17942824
  40. after diabetes, Protein kinase D control of LPL requires dissociation of Hsp25 from PKCdelta, association between PKCdelta and PKD, and vesicle fission. PMID: 18583709
  41. Cold acclimation significantly increased heparin-releasable LPL (P<0.05) and tissue residual LPL (P<0.01)in the heart. AMPK phosphorylation does not mediate the enhanced translocation of LPL to cardiac endothelium. PMID: 18722549
  42. Epididymal white adipose tissue perfused in situ with an nitric oxide donor rapidly releases LPL from the endothelium. PMID: 19088434

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Secreted
Protein Families AB hydrolase superfamily, Lipase family
Database Links

KEGG: rno:24539

STRING: 10116.ENSRNOP00000016543

UniGene: Rn.3834

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