Rat matrix metalloproteinase 10,MMP-10 ELISA kit

Code CSB-E07414r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
matrix metallopeptidase 10 (stromelysin 2)
Alternative Names
Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transformation-associated protein 34A ELISA Kit; Transin-2 ELISA Kit
Abbreviation
MMP10
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
80 pg/mL-2000 pg/mL
Sensitivity
50 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 85-99
1:2 Average % 92
Range % 90-103
1:4 Average % 95
Range % 87-105
1:8 Average % 95
Range % 87-99
Recovery
The recovery of rat MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 88-100
EDTA plasma (n=4) 95 89-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average
2000 0.172 0.175 0.174
800 0.376 0.379 0.378
480 0.554 0.562 0.558
200 0.882 0.897 0.890
80 1.493 1.475 1.484
0 2.404 2.452 2.428
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat MMP10 ELISA Kit was designed for the quantitative measurement of Rat MMP10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 80 pg/mL-2000 pg/mL and the sensitivity is 50 pg/mL.

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Target Background

Function
(From Uniprot)
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Gene References into Functions
  1. MMP-10 modulates corneal epithelial wound healing. PMID: 24339723
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Database Links
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