Rat matrix metalloproteinase 3,MMP-3 ELISA kit

Instructions
Code CSB-E07410r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Alternative Names Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
Abbreviation MMP3
Uniprot No. P03957
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %90
Range %87-93
1:200Average %94
Range %90-98
1:400Average %95
Range %91-99
1:800Average %92
Range %88-96
Recovery
The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8581-89
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.569 2.547 2.558 2.429
502.236 2.213 2.225 2.096
251.868 1.829 1.849 1.720
12.51.367 1.387 1.377 1.248
6.250.840 0.889 0.865 0.736
3.130.377 0.387 0.382 0.253
1.560.247 0.259 0.253 0.124
00.128 0.130 0.129  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Subcellular Location Secreted, extracellular space, extracellular matrix, Secreted
Protein Families Peptidase M10A family
Database Links

UniGene: Rn.32086

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