Rat microtubule-associated protein 2,MAP-2 ELISA Kit

Instructions
Code CSB-E12806r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name microtubule-associated protein 2
Alternative Names Map2 ELISA Kit; Mtap2 ELISA Kit; Microtubule-associated protein 2 ELISA Kit; MAP-2 ELISA Kit
Abbreviation MAP2
Uniprot No. P15146
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %86-93
1:2Average %103
Range %98-105
1:4Average %93
Range %88-97
1:8Average %97
Range %91-101
Recovery
The recovery of rat MAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-98
EDTA plasma (n=4)9792-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.329 2.312 2.321 2.185
51.844 1.776 1.810 1.674
2.51.274 1.296 1.285 1.149
1.250.923 0.917 0.920 0.784
0.6250.634 0.624 0.629 0.493
0.3120.433 0.443 0.438 0.302
0.1560.297 0.282 0.290 0.154
00.138 0.134 0.136  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Gene References into Functions
  1. selective axonal cargo trafficking requires the MAP2-defined pre-axonal filtering zone and the ability of cargos to switch between distinct kinesin motor activities PMID: 28426968
  2. dephosphorylation of MAP2D at Thr256 and Thr259, phosphorylation of vimentin at Ser38 and Ser72, and the resulting enhanced binding of MAP2D to vimentin might contribute to the progesterone synthetic response required for ovulation. PMID: 27335427
  3. This study demonstrated that motor skills training after ICH significantly improved motor and sensory functions as well as promoting MAP2 mRNA expression. PMID: 27265094
  4. Increased GluN2A, synaptophysin, BDNF, but not MAP2, observed after neonatal prebiotic feeding may suggest alteration in neurotransmission rather than synaptic architecture PMID: 26682524
  5. MAP2c/MAP2 could be one of the regulators maintaining tau homeostasis in the cell PMID: 26071842
  6. At the subcellular level CSI also showed significant impact on NFs and MAP-2 expression PMID: 25592752
  7. The obtained data provides the first atomic resolution insight into the conformational state of MAP2c. PMID: 23877929
  8. in neurons MARTA2 appears to represent a key trans-acting factor involved in dendritic targeting of MAP2 mRNAs. PMID: 23121659
  9. The effects of nerve growth factor (NGF) or neurotrophin-3 (NT-3) on the expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 was analyzed by real time-PCR assay. PMID: 22763971
  10. The brightness-area-product of MAP2 changes significantly across estrous cycle oscillations; it is twice as high in proestrus than in metestrus and estrus rats, and 1.5 times higher in proestrus than in diestrus rats. PMID: 21948316
  11. The expression of MAP-2 is strongly associated with the integrity of hippocampal neurons in young rats. Low-dose X-rays may promote the proliferation of hippocampal microtubule in hippocampus and further promote the expression of MAP-2. PMID: 22490839
  12. The MAP2 content only increased in the preoptic area at 24 h, and in the frontal cortex after 24 and 72 h of REM sleep deprivation. PMID: 22456537
  13. Serum levels of MAP2 remarkably increase as early as 0.5 hr following ischemia, much sooner than the observation of minimal tissue injury 3 hr following occlusion. PMID: 21948028
  14. morphological changes resulting from cortical compression may be related to reductions in MAP2-immunoreactive materials PMID: 21401443
  15. But MAP-2 neurons significantly decreased on just the 3rd day after chronic constriction injury. PMID: 21933624
  16. Progesterone decreased MAP2 content after a short-term treatment, while it increased that of MAP2 and TAU in this brain region after a long-term treatment. PMID: 21683086
  17. A transient increase in microtubule-associated protein (MAP)2 follows compressive injury of the cerebral cortex. PMID: 20568963
  18. The expression of MAP-2 mRNA gradually increased in the focus of the cerebral contusion from 12h to 14d after contusion. PMID: 20967947
  19. hyperbaric oxygenation treatment for cerebral infarction, can increase the expression of Map-2 and decrease the expression of GFAP. PMID: 19141977
  20. These results suggest that the involvement of repeated restraint stress alter the content and neuronal distribution of MAP2. PMID: 20513368
  21. Nitric oxide affects the phosphorylation state of microtubule-associated protein 2 in brain. PMID: 11829530
  22. In the developing telencephalon, high- and low-molecular-weight variants of MAP2 are differentially localized in a manner dependent on the process of maturation of the embryonic cortical neurons. PMID: 12115669
  23. In MAP2-deficient neurons, the induction of phosphorylated CREB after forskolin stimulation was much lower. MAP2 is an anchoring protein of protein kinase A in dendrites, whose loss reduces dendritic and total PKA and reduced CREB. PMID: 12163474
  24. Phosphorylation by protein kinase A within the proline-rich region of MAP2c at threonine-220 affects microtubule binding and stability PMID: 12369833
  25. MAP2 protein content is significantly increased in the hippocampus following administration of both estradiol and progesterone. PMID: 12372566
  26. Results support the existence of regions of nascent structure in the N-terminal region of microtubule-associated protein 2 (MAP2), which are believed to contribute to its regulatory function. PMID: 12535652
  27. After brain ischemia, MAP2 immunoreactivity was significantly reduced in the cerebral cortex and hippocampus. PMID: 12757367
  28. The proteolysis of MAP2 is limited by endogenous calpain activity, likely via the spatial access of calpain to MAP2. PMID: 12834896
  29. The level of Map2 and the rate of its phosphorylation were increased in neurons of this inbred strain (in comparison to Wistar rats). Daily kindling further decreased the length of microtubule fragments. PMID: 14989172
  30. activity regulated cytoskeletal-associated proteininteracted with newly polymerized microtubules and microtubule-associated protein 2, leading to blocking of the epitope of microtubule-associated protein 2 PMID: 15048929
  31. GAP-43 and MAP-2 in the brain have roles in oxidative stress and aging PMID: 15246990
  32. We suggest that changes in MAP2 are involved in the structural changes that occur in the hippocampus of the rat during the estrous cycle, and that these variations are related to changes in estradiol and progesterone levels. PMID: 15307151
  33. Cognitive impairment and the expression patterns of MAP-2, GAP-43, and synaptophysin were examined in rats with permanent occlusion of bilateral common carotid arteries. PMID: 15964096
  34. JNK1 phosphorylation of MAP2 plays an important role in defining dendritic architecture in the brain. PMID: 16000625
  35. Neonatal treatment with pregnenolone and DHEA increased the expression of MAP-2 in the rat hippocampus and nucleus accumbens but not in the prefrontal cortex, striatum or amygdala in adulthood. PMID: 16002213
  36. Stimulatory effects of pregnenolone and 3beta-methoxypregnenolone on neurite extension show that MAP2 is neurosteroid specific receptor. PMID: 16537405
  37. MAP2 is involved in ERK3 mediated insulin secretion PMID: 16597486
  38. Within the somatosensory cortex, MK801 triggered robust, caspase-3-dependent apoptotic injury, reduced expression of cytoskeletal proteins MAP2 and tau, and increased synapse associated protein SNAP25. PMID: 16708014
  39. TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites PMID: 16901895
  40. Expression of MAP2 does not seem to indicate difficulty of task or duration of training time. PMID: 17141532
  41. The strictly developmentally regulated MAP2 isoform expression suggests different functional roles for these proteins during the postnatal development in the rat hippocampus. PMID: 17229541
  42. Prolactin increased the association between SNARE proteins and kinesin/MAP-2 while the association of munc-18/syntaxin 1A was decreased. PMID: 17573185
  43. In PC12 cells, epinephrine induces phosphorylation of MAP-2c at ser136 through a alpha 2-adrenoceptor mediated, ERK/PKC-dependent signaling pathway, which may contribute to the stabilization of neurites. PMID: 18290605
  44. Gap-43 co-localizes and potentially interacts with microtubule-associated protein MAP-2 in adult and fetal rat brain, as well as in primary neuronal cultures. PMID: 18455509
  45. Progesterone maintained in part neuronal ultrastructure, attenuated chromatolysis, and preclude the loss of MAP2, suggesting a protective effect during the early phases of spinal cord injury. PMID: 18584320
  46. data reveal occurrence of interactions between microtubule polymers involving MAP2 and modulated by cAMP; findings imply role for the complex of cAMP-dependent protein kinase A with the projection domain of MAP2 in microtubule-microtubule interactions PMID: 19009287
  47. Protein kinase A was concentrated in dendritic shafts; this distribution was imposed by the microtubule-binding protein MAP2, indicating that MAP2 is the dominant A-kinase anchoring protein in neurons. PMID: 19447092

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Subcellular Location Cytoplasm, cytoskeleton, Cell projection, dendrite
Database Links

STRING: 10116.ENSRNOP00000050877

UniGene: Rn.10484

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