Rat periostin/osteoblast specific factor 2 (POSTN) ELISA kit

Code CSB-EL018381RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat POSTN ELISA Kit was designed for the quantitative measurement of Rat POSTN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.81 ng/mL.

Target Name periostin/osteoblast specific factor 2 (POSTN)
Abbreviation POSTN
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %86-96
1:2Average %104
Range %98-108
1:4Average %90
Range %85-94
1:8Average %98
Range %92-101
The recovery of rat POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9184-95
EDTA plasma (n=4)9890-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
20002.565 2.487 2.526 2.343
10002.140 2.078 2.109 1.926
5001.383 1.369 1.376 1.193
2500.842 0.804 0.823 0.640
1250.475 0.507 0.491 0.308
62.50.417 0.403 0.410 0.227
31.250.280 0.272 0.276 0.093
00.185 0.181 0.183
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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