Rat periostin/osteoblast specific factor 2 (POSTN) ELISA kit

Instructions
Code CSB-EL018381RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name periostin/osteoblast specific factor 2 (POSTN)
Abbreviation POSTN
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %86-96
1:2Average %104
Range %98-108
1:4Average %90
Range %85-94
1:8Average %98
Range %92-101
Recovery
The recovery of rat POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9184-95
EDTA plasma (n=4)9890-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
20002.565 2.487 2.526 2.343
10002.140 2.078 2.109 1.926
5001.383 1.369 1.376 1.193
2500.842 0.804 0.823 0.640
1250.475 0.507 0.491 0.308
62.50.417 0.403 0.410 0.227
31.250.280 0.272 0.276 0.093
00.185 0.181 0.183
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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