Rat platelet activating factor,PAF ELISA KIT

Instructions
Code CSB-E07930r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name platelet activating factor,PAF
Alternative Names N/A
Abbreviation PAF
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Biochemicals
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 92-104  
1:2 Average % 92  
Range % 87-98  
1:4 Average % 89  
Range % 86-98  
1:8 Average % 104  
Range % 96-108  
Recovery
The recovery of rat PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 80-92  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.611 2.710 2.661 2.544  
50 2.058 2.157 2.108 1.991  
25 1.487 1.506 1.497 1.380  
12.5 0.899 0.908 0.904 0.787  
6.25 0.466 0.457 0.462 0.345  
3.12 0.290 0.281 0.286 0.169  
1.56 0.182 0.187 0.185 0.068  
0 0.118 0.115 0.117    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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