Rat procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Code CSB-E12774r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name procollagen Ⅰ N-terminal peptide,PⅠNP
Alternative Names procollagen 1 N-terminal peptide,P1NP, procollagen I N-terminal peptide,PINP
Abbreviation PⅠNP
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 100  
Range % 95-105  
1:10 Average % 96  
Range % 90-102  
1:20 Average % 97  
Range % 92-108  
1:40 Average % 96  
Range % 90-103  
The recovery of rat PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-103  
EDTA plasma (n=4) 100 95-106  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.488 2.527 2.508 2.331  
2000 1.848 1.839 1.844 1.667  
1000 1.331 1.356 1.344 1.167  
500 0.909 0.963 0.936 0.759  
250 0.524 0.534 0.529 0.352  
125 0.314 0.329 0.322 0.145  
62.5 0.254 0.267 0.261 0.084  
0 0.176 0.178 0.177    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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