Rat von Willebrand Factor,vWF ELISA Kit

Code CSB-E08438r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
von Willebrand factor
Alternative Names
Vwf ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit; Fragment ELISA Kit
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
0.078 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 86-99  
1:200 Average % 97  
Range % 92-105  
1:400 Average % 85  
Range % 81-87  
1:800 Average % 88  
Range % 83-95  
The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-93  
EDTA plasma (n=4) 95 90-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.300 2.488 2.394 2.257  
10 2.011 2.102 2.057 1.920  
5 1.446 1.560 1.503 1.366  
2.5 0.903 0.900 0.902 0.765  
1.25 0.628 0.586 0.607 0.470  
0.625 0.350 0.364 0.357 0.220  
0.312 0.242 0.258 0.250 0.113  
0 0.134 0.140 0.137    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The Rat von Willebrand Factor, vWF ELISA Kit is a highly sensitive and reliable assay for the quantification of von Willebrand factor in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat).

von Willebrand factor is an important protein involved in the formation of blood clots and is crucial in the prevention of excessive bleeding. Our ELISA kit is designed to accurately measure the levels of von Willebrand factor in biological samples, making it an essential tool for researchers in the field of cardiovascular research.

The detection range of the kit is 0.312 ng/mL-20 ng/mL, with a sensitivity of 0.078 ng/mL, ensuring precise and accurate quantification of von Willebrand factor in samples. The assay time is only 1-5 hours, and the sample volume required is minimal (50-100ul), making the kit highly efficient and suitable for high-throughput experiments.

The vWF ELISA kit uses a sandwich assay principle, which involves the use of a vWF-specific antibody to capture the protein of interest, followed by the detection of the captured von Willebrand factor using a second vWF-specific antibody. The detection wavelength is 450 nm, and the results are easily obtained using a microplate reader.

This vWF ELISA kit has been extensively validated, with over 13 citations in scientific literature, demonstrating its reliability and accuracy in detecting von Willebrand factor in biological samples.


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Target Background

(From Uniprot)
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Subcellular Location
Secreted. Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Database Links
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