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| Code | CSB-E08438r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-99 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-87 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 83-95 | |||||
| The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-93 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat von Willebrand Factor, vWF ELISA Kit is a highly sensitive and reliable assay for the quantification of von Willebrand factor in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat).
von Willebrand factor is an important protein involved in the formation of blood clots and is crucial in the prevention of excessive bleeding. Our ELISA kit is designed to accurately measure the levels of von Willebrand factor in biological samples, making it an essential tool for researchers in the field of cardiovascular research.
The detection range of the kit is 0.312 ng/mL-20 ng/mL, with a sensitivity of 0.078 ng/mL, ensuring precise and accurate quantification of von Willebrand factor in samples. The assay time is only 1-5 hours, and the sample volume required is minimal (50-100ul), making the kit highly efficient and suitable for high-throughput experiments.
The vWF ELISA kit uses a sandwich assay principle, which involves the use of a vWF-specific antibody to capture the protein of interest, followed by the detection of the captured von Willebrand factor using a second vWF-specific antibody. The detection wavelength is 450 nm, and the results are easily obtained using a microplate reader.
This vWF ELISA kit has been extensively validated, with over 13 citations in scientific literature, demonstrating its reliability and accuracy in detecting von Willebrand factor in biological samples.
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STRING: 10116.ENSRNOP00000026643
UniGene: Rn.35561
