Rat von Willebrand Factor,vWF ELISA Kit

Instructions
Code CSB-E08438r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name von Willebrand factor
Alternative Names Vwf ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit; Fragment ELISA Kit
Abbreviation VWF
Uniprot No. Q62935
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 86-99  
1:200 Average % 97  
Range % 92-105  
1:400 Average % 85  
Range % 81-87  
1:800 Average % 88  
Range % 83-95  
Recovery
The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-93  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.300 2.488 2.394 2.257  
10 2.011 2.102 2.057 1.920  
5 1.446 1.560 1.503 1.366  
2.5 0.903 0.900 0.902 0.765  
1.25 0.628 0.586 0.607 0.470  
0.625 0.350 0.364 0.357 0.220  
0.312 0.242 0.258 0.250 0.113  
0 0.134 0.140 0.137    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma (By similarity).
Subcellular Location Secreted, Secreted, extracellular space, extracellular matrix
Tissue Specificity Plasma.
Database Links

STRING: 10116.ENSRNOP00000026643

UniGene: Rn.35561

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