Sheep Follicle Stimulating Hormone (FSH) ELISA kit

Instructions
Code CSB-E13170Sh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Alternative Names FSHBFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
Abbreviation FSH
Uniprot No. P01227
Species Ovis aries (Sheep)
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-94  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 94  
Range % 90-99  
1:8 Average % 92  
Range % 87-96  
Recovery
The recovery of sheep FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-101  
EDTA plasma (n=4) 95 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
70 1.543 1.476 1.510 1.454  
25 0.732 0.715 0.724 0.668  
10 0.397 0.402 0.400 0.344  
5 0.258 0.253 0.256 0.200  
2 0.113 0.111 0.112 0.056  
0 0.054 0.058 0.056    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Gene References into Functions
  1. A novel GnRH response element between -750 bp and -232 bp of the ovine FSHB promoter. PMID: 23874399
  2. Data suggest that dietary components (phytoestrogens here) down-regulate basal secretion/total FSH (CGA+FSHB) production yet up-regulate gonadotropin-releasing hormone agonist-induced luteinizing hormone (CGA+LHB) secretion in gonadotrophs. PMID: 24050980
  3. In ewes, infusion with genistein decreased the level FSH-beta in the adenohypophysis. PMID: 15531133
  4. Activation of TAK1 (and probably p38-MAPK), but not Smad3, is necessary for triggering induction of oFSHbeta by activin. PMID: 16081641
  5. Activin gathers Smads and Smad-associated proteins at the promoter region to regulate expression of the ovine FSHB and overall follitropin production. PMID: 17584958

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Subcellular Location Secreted.
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: oas:443387

UniGene: Oar.430

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