Sheep Follicle Stimulating Hormone (FSH) ELISA kit

Code CSB-E13170Sh
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01227
Lead Time 3-5 working days
Abbreviation FSH
Protein Biological Process 1 Sex hormone
Target Name follicle stimulating hormone, beta polypeptide
Alias , follicle-stimulating hormone beta subunit|follitropin, beta chain
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 2 mIU/mL-70 mIU/mL
Sensitivity 1 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein.
HGNC 3964
RGD 2630
MGI 95582
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-94  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 94  
Range % 90-99  
1:8 Average % 92  
Range % 87-96  
Recovery
The recovery of sheep FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-101  
EDTA plasma (n=4) 95 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
70 1.543 1.476 1.510 1.454  
25 0.732 0.715 0.724 0.668  
10 0.397 0.402 0.400 0.344  
5 0.258 0.253 0.256 0.200  
2 0.113 0.111 0.112 0.056  
0 0.054 0.058 0.056    
References
Still Have Questions? Leave a Message or Start an on-line Chat
Function Stimulates development of follicle and spermatogenesis in the reproductive organs.
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: oas:443387

UniGene: Oar.430

Pathway cAMP signaling pathway

Related Products

FSHB Proteins

FSHB Proteins for Ovis aries (Sheep)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.